RPMI-1640 was developed by Moore, et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

Powder with uniform and free flowing appearance from pink to orange

Solubility Magenta, clear, complete

pH7.9±0.9

Endotoxin ≤ 1 unit/ml

For each liter, dissolve 10.4g in 900ml ddH2O, and stir until completely dissolved. Do not heat. If necessary, add 2g sodium bicarbonate with stirring. Adjust the pH value of the medium to 0.1-0.3 pH units lower than the required pH value. Add additional water to bring the solution to its final volume. Filter and disinfect with 0.22um membrane. Divide equally into sterile containers.

RPMI-1640廣泛應(yīng)用于哺乳動(dòng)物、特殊造血細(xì)胞、正常或惡性增生的白細(xì)胞，雜交瘤細(xì)胞的培養(yǎng)，是目前應(yīng)用十分廣泛的培養(yǎng)基。主要用于懸浮細(xì)胞培養(yǎng)。其它像K-562、HL-60、Jurkat、Daudi、IM-9等成淋巴細(xì)胞、T細(xì)胞淋巴瘤細(xì)胞以及HCT-15上皮細(xì)胞等均可參考使用。

RPMI-1640培養(yǎng)基已用于培養(yǎng)人正常和腫瘤白細(xì)胞。當(dāng)適當(dāng)補(bǔ)充RPMI-1640時(shí)，已證明其廣泛適用于支持多種類型的培養(yǎng)細(xì)胞的生長(zhǎng)，包括72小時(shí)植物血凝素（PHA）刺激試驗(yàn)中的新鮮人淋巴細(xì)胞。

艾美捷RPMI-1640培養(yǎng)基L-谷丨氨酰胺含L-谷丨氨酰胺和25mMHEPES，不含碳丨酸氫鈉，粉末形式。

英文名字：RPMI 1640, With L-glutamine and 25 mM HEPES. Without sodium bicarbonate.

外觀：粉色至橙色均勻、自由流動(dòng)的粉末

pH：7.9±0.9

內(nèi)毒素：≤1EU/ml

等級(jí)：細(xì)胞培養(yǎng)等級(jí)

儲(chǔ)存溫度：室溫/4℃

相關(guān)文獻(xiàn)：

1. Moore, G.E., Gerner, R.E., Franklin, H.A., JAMA 199: 519-524 (1967). 2. Moore, G.E., Woods L.K., Tissue Culture Association Manual 3: 503-508. (1976). 3. Moore, G.E. Gerner, R.E., Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research 41-63. (1967, February). 4. Moore, G.E. Gerner, R.E., Kitamura,H., NY State Journal of Medicine 68: 2054-2060 (1968).