Staurosporine is a natural product derived alkaloid which is isolated from Streptomyces staurospores cultures. This protein kinase inhibitor belongs to a family of kinase inhibitors containing an indole carbozol chromophore and is a potent inhibitor of a number of kinases. The ATP binding site on these kinase enzymes appears to be targeted by these Streptomyces derived inhibitors. Inhibition of these intracellular kinases leads to the induction of apoptosis as exhibited by the classic chromatin condensation leading to the formation of micronuclear bodies and reduced cell volumes. DNA is subsequently cleaved into oligonucleosomal fragments (laddering) as evidenced by agarose gel analysis. Staurosporine can be used with ICT’s apoptosis and cytotoxicity detection assays to create positive controls.

Reagent Name Staurosporine

Target Kinases

Sample Type Cell culture

Storage -20°C

星狀孢菌素是從星狀孢鏈霉菌培養(yǎng)物中分離的天然產物衍生的生物堿。這種蛋白激酶抑制劑屬于含有吲哚羧唑發(fā)色團的激酶抑制劑家族，是多種激酶的有效抑制劑。這些激酶酶上的ATP結合位點似乎是這些鏈霉菌衍生的抑制劑的目標。抑制這些細胞內激酶可誘導細胞凋亡，典型的染色質凝結可導致微核體的形成和細胞體積的減少。隨后，瓊脂糖凝膠分析證實DNA被裂解成寡核體片段(梯形)。nstaaurosporine可用于ICT的凋亡和細胞毒性檢測，以建立陽性對照。

艾美捷星形孢菌素Staurosporine是從stauro孢子鏈霉菌中分離出來的一種蛋白激酶抑制劑。Staurosporine在1µM的2 ~ 3 h內誘導DNA破裂和凋亡。

艾美捷星形孢菌素Staurosporine基本操作：

1、將十字孢粉溶解在組織培養(yǎng)級DMSO中，以獲得1mM十字孢粉濃度。

2、制備50–100µL的DMSO增溶星孢菌素儲備溶液等分試樣，并將其冷凍保存在<-20°C。一瓶冷凍的星孢菌素在必須丟棄之前只能重新包裝2次。

3、細胞培養(yǎng)基中星形孢菌素濃度為1µM的刺突細胞培養(yǎng)物。這相當于每毫升細胞培養(yǎng)懸浮液中1µL的1mM十字孢菌素原液。當使用4-5小時37°C孵育期時，該濃度對于誘導包括HL-60和Jurkat細胞在內的許多細胞系非常有效。用于該星孢菌素誘導方案的典型細胞懸浮液濃度范圍為1 x 105–1 x 106細胞/mL。

4、對特定細胞系進行時間進程研究，以確定在實驗系統(tǒng)中實現(xiàn)良好的凋亡誘導水平所需的合適的星孢菌素濃度和暴露時間。

艾美捷星形孢菌素Staurosporine相關研究：

Recombinant Mouse IL-1 alpha

Recombinant Human IL-8

NIR-FLIVO 690 Free Dye Control Assay

Antibody-Sandwich ELISA Development Kit

ELISA Plate Sealing Covers

Monster Block ELISA Blocking Buffer

Foil Storage Bags for ELISA Plates

Sample Diluent Optimization Pack

Pack of Desiccant Packets

重組小鼠IL-1α

重組人IL-8

NIR-FLIVO 690游離染料對照試驗

抗體夾心ELISA開發(fā)試劑盒

ELISA板密封蓋

Monster Block ELISA阻斷緩沖液

ELISA板用箔存儲袋

樣品稀釋劑優(yōu)化包

干燥劑包裝