Synthesis and structure

Blue-dextrans are synthesized from controlled dextran fractions by reacting with Cibacron blue F3GA. After purification from non-bound dye, the products are controlled for mean weight average molecular weight (Mw), solubility, degree of substitution, free dye and loss on drying. The low molecular weight blue-dextrans (BD5, BD10 and BD20) require purification by chromatographic methods during production and are therefore more expensive than the larger ones.

The products are designated by the approximate molecular weights of the dextran fractions used. Thus, for example, the product BD5 has a molecular weight of approx. 5000. The actual molecular weight is determined by gel permeation chromatography (GPC). This value is supplied with the Certificate of Analysis. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear α-(1-6)-linked glucose chain with a low percentage (2-5%) of α-(1-3) branches distributed along the chain. The dextran fractions used are from weight average molecular weights (Mw) of 4000 to 2000000 and are carefully controlled by GPC, optical rotation, absorbance and other control parameters

藍色葡聚糖2000長期以來一直被用作色譜法中的空隙體積標記物，并且用于色譜 法的藍色-葡聚糖凝膠綴合物已經使用多年（例如參見GE Healthcare Life Sciences；分子排阻色譜法；原理和方法）。使用藍色葡聚糖的其它重要研究領域包括：

溶酶體活性的研究

內皮細胞通透性

牛精子滲透性

角膜通透性

肺血流研究

腦通透性

蛋白質和酶與藍色葡聚糖的結合

藍色葡聚糖是通過與Cibacron藍F3GA反應由受控右旋糖聚糖部分合成的。從非結合染料中純化后，對產物的平均重量進行控制分子量（Mw）、溶解度、取代度、游離染料和干燥損失。低點分子量藍色藍色葡聚糖（BD5、BD10和BD20）需要通過色譜純化方法，因此比較大的方法更昂貴。產品由所用葡聚糖部分的近似分子量指-定。

因此，例如，產物BD5具有大約5000的分子量。實際分子通過凝膠滲透色譜法（GPC）測定重量。此值隨分析證書。所使用的葡聚糖來自間質明串珠菌B-512F基本上是線性α-（1-6）連接的葡萄糖鏈，具有低百分比（2-5%）的α-（1-3）分支沿著鏈條分布。所使用的葡聚糖組分來自重均分子量（Mw）為4000至2000000，并通過GPC、旋光度、吸光度和其他仔細控制控制參數(shù)。

艾美捷Blue dextran（藍色葡聚糖）#TDB-BD5、BD10和BD20：是一種藍色無味粉末，可自由溶解于水或電解質溶液中。由于存在兩種磺酸鹽，該產品具有明顯的兩性特征以及Cibacron Blue中的氨基。由于這種性質可能會影響溶液中的流體動力學體積以及與凝膠的相互作用，因此在GPC上運行樣品時發(fā)現(xiàn)了一些異常。

藍色葡聚糖不溶于大多數(shù)有機溶劑，例如乙醇、甲醇、丙酮，氯仿、乙酸乙酯等。藍色葡聚糖110在水中的吸收光譜所使用的發(fā)色團在621.5nm處具有最大吸光度。

文獻參考：

1. D.M.Wolfe, J.H.Lee, A.Kumar et al., Autophagy failure in Alzheimers disease and role of defective lysosomal acidification, Eur.J.Neurosci., 2013,37(12), 1949-61.

2. C.M.Waters, J.S.Alexander, T.R.Harris et al., Perilla ketone increases endothelial cell monlayer in vitro, J.Appl.Physiol., 1993,74(5), 24932501

3. P.K.Schoff and N.L.First, Stimulation of bovine cell motility by the triazine dye cibacron blue F3GA, Mol.Reprod.dev., 1995,42(1), 65-71.

4. R.M.Robbins and M.A.Galin, A model for steroid effects in herpes keratitis, Arch.Ophthalmol., 1975,93(9),828-30.

5. S.T.Ballard and J.T.Gatzy, Volume flow across the alveolar epithelium of adult rat lung, J.Appl.Physiol., 1991,70(4),1665-16676.

6. S.Cassin, Effect of indomethacin on fetal lung liquid formation, Can.J.Physiol.Pharmacol., 1984,62(1),157-9

7. R.E.Pfister, C.A.Ramsden, H.L.Neil et al., Errors in estimating lung liquid in fetal lambs when using radiolabelled serum albumin and blue dextran, J.Appl.Physiol., 1999, 87(6) 2366-74

8. C.E.Johanson, Age-related decrease inA-Beta Peptide clearance pathways: CSF and BBB, RePORTER (Database NIH),

9. S.S.Prabhu, Broaddus, G.T.Gillies et al., Distribution of macromolecular dyes in brain using positive pressure. Surg.Neurol., 1998, 50(40) 367-375.

10. L.Z.Bito, H.Davidson and J.R.Hollingsworth, Facilitated transport of prostaglandins across the blood-cerebrospinal fluid an blood-brain barrier, J.Physiol., 1976, 256(2), 273-85

11. Reshma SV1, Sathyanarayanan N, Nagendra HG, Characterization of hypothetical protein VNG0128C from Halobacterium NRC-1 reveals GALE like activity and its involvement in Leloir pathway of galactose metabolism, J Biomol Struct Dyn. 2014 Nov 14:1-13.

12. G.E.J.Staal, J.F.Koster, H.Kamp et al., Human erythrocyte pyruvate kinase. Its purification and some properties, Biochim.Biophys.Acta, 1971, 227, 86-89

13. A.C.W.Swart and H.C.Hemker, Separation of blood coagulation factors II,VII,IX and X by gel filtration in the presence of Dextran blue, Biochim.Biophys.Acta, 1970, 222, 692-695