In vivo SilenceMag™ is a rapid, simple and highly efficient method dedicated to transfect small RNA (siRNA, miRNA) into target cells/tissue in vivo. It combines magnetic nanoparticles and small RNA that will be retained after injection at the magnetically targeted site. This targeted delivery method minimizes systemic distribution, increases gene targeted inactivation and reduces toxicity. Furthermore, the magnetic forces enhance the uptake of magnetic nanoparticles by the target tissue, and thus improve the efficiency of silencing.

This allows decreasing the required process time of delivery to few minutes which is crucial for improvement of in vivo small RNA delivery. In vivo SilenceMag™ is designed to meet in vivo grade quality.

In vivo SilenceMag™ benefits:

Increased silencing efficiency

Targeted process (magnetically-driven)

Reduction of the systemic dissemination of siRNA/miRNA during injection

Reduction of the siRNA/miRNA doses

Work under non permissive conditions (hypothermia, physiological flow conditions)

Penetration of the siRNA/miRNA into tissues

Minimized toxicity

艾美捷IN VIVO SILENCEMAG TRIAL試劑盒#IV-TK30240IN VIVO SILENCEMAG TRIAL試劑盒是一種快速、簡單且高效的方法，致力于將小 RNA（siRNA、miRNA）轉(zhuǎn)染到體內(nèi)靶細胞/組織中。它結(jié)合了磁性納米顆粒和小RNA，在磁性靶向位點注射后將保留。這種靶向遞送方法極大限度地減少了全身分布，增加了基因靶向失活并降低了毒性。此外，磁力增強了靶組織對磁性納米顆粒的吸收，從而提高了SILENCEMAG效率。

這樣可以將所需的遞送過程時間減少到幾分鐘，這對于改善體內(nèi)小RNA遞送至關(guān)重要。體內(nèi)SilenceMag™旨在滿足體內(nèi)級質(zhì)量。

IN VIVO SILENCEMAG TRIAL試劑盒特點：

提高消音效率

目標(biāo)工藝（磁力驅(qū)動）

減少注射過程中siRNA/miRNA的全身性傳播

減少siRNA/miRNA劑量

在非許可條件下工作（體溫過低、生理流動條件）

siRNA/miRNA滲透到組織中

毒性最小化

IN VIVO SILENCEMAG TRIAL試劑盒結(jié)果示例：

小鼠皮下腫瘤轉(zhuǎn)染。 Chen和她的合作者已經(jīng)表明，使用體內(nèi)SilenceMag與外部磁場的應(yīng)用相結(jié)合，增加了針對腫瘤部位血管內(nèi)皮生長因子（VEGF）的siRNA的吸引力和保留。通過SPECT和MRI評估siRNA / SilenceMag的生物分布和細胞毒性，并每天評估腫瘤大?。–hen T，Zhu S，Tong L，Li J，Chen F，Han Y，Zhao Y，Xiong W. 2014 超順磁性氧化鐵納米顆粒介導(dǎo)的131I-hVEGF siRNA抑制裸鼠肝細胞癌腫瘤生長。BMC 癌癥。14:414).

圖：通過將肝癌腫瘤細胞注射到免疫抑制小鼠的右脅中產(chǎn)生皮下腫瘤。然后每天在靜脈注射VEGF / SilenceMag后監(jiān)測腫瘤生長。來自Chen等人，BMC Cancer，2014;14:114.