英文名稱  Anti-DIS3
中文名稱  有絲分裂控制蛋白dis3抗體
別    名  Dis3; DIS3 mitotic control homolog (S. cerevisiae); dis3p; Exosome complex exonuclease RRP44; Mitotic control protein dis3 homolog; Protein DIS3 homolog; Ribosomal RNA processing protein 44; Ribosomal RNA-processing protein 44; RRP44; RRP44_HUMAN.
應(yīng)用于醫(yī)學(xué)免疫學(xué)、動(dòng)物免疫學(xué)、分子生物學(xué)、生物化學(xué)、臨床醫(yī)學(xué)、檢驗(yàn)醫(yī)學(xué)、動(dòng)物醫(yī)學(xué)、藥學(xué)、理工、農(nóng)學(xué)、環(huán)境學(xué)等生命科學(xué)密切相關(guān)的專業(yè)。         
濃    度  1mg/1ml
規(guī) 格  0.2ml/200μg         
抗體來源  Rabbit 
克隆類型  polyclonal
交叉反應(yīng)  Human, Mouse, Rat, Dog, Horse, Rabbit
產(chǎn)品類型  一抗   
研究領(lǐng)域  細(xì)胞生物 細(xì)胞周期蛋白 細(xì)胞分化 表觀遺傳學(xué) 
蛋白分子量  predicted molecular weight: 105kDa
性    狀  Lyophilized or Liquid
免 疫 原  KLH conjugated synthetic peptide derived from human DIS3 (701-730aa)
亞    型  IgG
純化方法  affinity purified by Protein A
儲 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
（石蠟切片需做抗原修復(fù)）
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user. 
保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

有絲分裂控制蛋白dis3抗體產(chǎn)品介紹 Component of the exosome that has both 3'-5' exonuclease and endonuclease activities. Required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. As part of nucleoplasmic and probably cytoplasmic forms of the RNA exosome complex, it is involved in RNA processing and turnover. Also associated with the GTPase RAN.

Function : Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.

Subunit : Component of the RNA exosome complex. The catalytically inactive RNA exosome core (Exo-9) complex is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic-and nuclear-specific RNA exosome complex forms.

Subcellular Location : Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Note=Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3.
Tissue Specificity : Widely expressed.
Similarity : Belongs to the RNR ribonuclease family.
Contains 1 PINc domain.

【存儲要求】連續(xù)使用時(shí)4°C存儲，保質(zhì)期六個(gè)月；有絲分裂控制蛋白dis3抗體期存儲時(shí)建議分裝為10ul以上小包裝-20°C存儲，并避免反復(fù)凍融，保質(zhì)期一年。

實(shí)驗(yàn)的用途：
1）WB：Western Blotting 免疫印跡
2）IH：Immunohistochemistry 免疫組化
3）IH(P)：Immunohistochemistry Parraffin sections 免疫組化(石蠟)
4）IH(F)：Immunohistochemistry Frozen sections 免疫組化(冰凍)
5）IC：Immunocytochemistry 免疫細(xì)胞化學(xué)