中文名稱    細(xì)胞毒性T細(xì)胞抗原-4抗體    
別    名    CD 152; CD152; CD152 antigen; Celiac disease 3; CELIAC3; CTLA 4; CTLA-4; Cytotoxic T cell associated 4; Cytotoxic T lymphocyte associated 4; Cytotoxic T lymphocyte associated antigen 4; Cytotoxic T lymphocyte associated protein 4; Cytotoxic T lymphocyte associated serine esterase 4; Cytotoxic T lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; Cytotoxic T-lymphocyte-associated protein 4; GSE; IDDM12.      
Specific References  (1)     |     bs-10006R has been referenced in 1 publications.
[IF=4.12] Seike, Masahiro, et al. "Histamine suppresses regulatory T cells mediated by TGF‐β in murine chronic allergic contact dermatitis." Experimental Dermatology(2015).  IHC-F ;  Mouse.  
PubMed:25651189
研究領(lǐng)域    
抗體來源    Rabbit    
克隆類型    Polyclonal    
交叉反應(yīng)    Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep,     
產(chǎn)品應(yīng)用    WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg/Test  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    
分 子 量    21kDa    
細(xì)胞定位    細(xì)胞膜 細(xì)胞外基質(zhì)     
性    狀    Lyophilized or Liquid    
濃    度    1mg/ml    
免 疫 原    KLH conjugated synthetic peptide derived from human CTLA-4/CD152:75-170/223 <Extracellular>    
亞    型    IgG    
純化方法    affinity purified by Protein A    
儲 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    
保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    
PubMed    PubMed    
產(chǎn)品介紹    background:
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
抗原抗體結(jié)合特點
 1）特異性：抗原分子只能與由它刺激所產(chǎn)生的抗體結(jié)合而起反應(yīng)的專一性能；
 2）可逆性：是指Ag與相應(yīng)Ab結(jié)合成IC后，在一定條件下可解離為游離抗原與抗體。
 3）階段性：
 一階段：特異性結(jié)合階段 （①反應(yīng)速度快，幾秒鐘或幾分鐘即可完成 ②不出現(xiàn)肉眼可見反應(yīng)）；
 第二階段：可見階段（表現(xiàn)為凝集、沉淀、補(bǔ)體結(jié)合等反應(yīng) ①反應(yīng)進(jìn)行慢，需要幾分鐘、幾十分鐘或geng長②受電解質(zhì)、溫度、酸堿度等多種因素影響）。
4）比例性：（zui適比或等價點）是指抗原與抗體發(fā)生可見反應(yīng)需遵循一定的量比關(guān)系，只有當(dāng)二者濃度比例適當(dāng)時，才出現(xiàn)可見反應(yīng)。
抗原抗體反應(yīng)影響因素：
（一）反應(yīng)物自身的因素
5） 抗體：不同來源的抗體,反應(yīng)性各有差異,抗體的濃度、特異性和親和力都影響抗體抗原反應(yīng),為提高試驗的可靠性,應(yīng)選擇高特異性、高親和力的抗體作診斷試劑.等價帶的寬窄也影響抗原抗體復(fù)合物的形成,單克隆抗體不適用于沉淀反應(yīng).
6）  抗原：抗原的理化性狀、分子量、抗原決定簇的種類及數(shù)目均可影響反應(yīng)結(jié)果.顆粒性抗原出現(xiàn)凝集反應(yīng),可溶性抗原出現(xiàn)沉淀反應(yīng),單價抗原與相應(yīng)抗體結(jié)合不出現(xiàn)沉淀現(xiàn)象.
（二）反應(yīng)環(huán)境條件
7） 酸堿度：抗原抗體反應(yīng)必須在合適的pH環(huán)境中進(jìn)行.蛋白質(zhì)具有兩性電離性質(zhì),因此每種蛋白質(zhì)都有固定的等電點.抗原抗體反應(yīng)一般在pH6～9進(jìn)行,有補(bǔ)體參與的反應(yīng)pH為7.7.4,pH過高或過低都將影響抗原與抗體反應(yīng).
8）  溫度：在一定范圍內(nèi),溫度升高可加速分子運(yùn)動,抗原與抗體碰撞機(jī)會增多,使反應(yīng)加速.一般為15℃～40℃,常用的抗原抗體反應(yīng)溫度為37℃,溫度如高于56℃,可導(dǎo)致已結(jié)合的抗原抗體再解離,甚至變性或破壞.每種試驗都有其*的zui適反應(yīng)溫度要求.此外,適當(dāng)振蕩也可促進(jìn)抗原抗體分子的接觸,加速反應(yīng).