試劑盒名稱:非神經(jīng)元性烯醇化酶(NNE)

不對稱二甲基*

規(guī)格: 96T/48T
品牌:BIOFINE
種屬:人ELISA試劑盒
檢測波長:450nm
所需樣本體積: 50-100ul
適用范圍:僅供科研
保存及有效期:2-8℃，六個月，-20℃一年
檢測目的:用于測定血清，血漿及相關(guān)液體非神經(jīng)元性烯醇化酶(NNE)含量。適合檢測包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等標(biāo)本。 

contains the standard concentration of analyte will be prepared. Unknowns that generate a signal that is stronger than the known sample are "positive." Those that generate weaker signal are "negative." Doctor Dennis E Bidwell and Alister Voller created the test. History Before the development of the ELISA, the only option for conducting an immunoassay was radioimmunoassay, a technique using radioactively-labeled antigens or antibodies. In radioimmunoassay, the radioactivity provides the signal, which indicates whether a specific antigen or antibody is present in the sample. Radioimmunoassay was first described in a paper by Rosalyn Sussman Yalow and Solomon Berson published in 1960.[5] Because radioactivity poses a potential health threat, a safer alternative was sought. A suitable alternative to radioimmunoassay would substitute a non-radioactive signal in place of the radioactive signal. When enzymes (such as peroxidase) react with appropriate substrates (such as ABTS or 3,3’,5,5’-Tetramethylbenzidine), a change in color occurs, which is used as a signal. However, the signal has to be associated with the presence of antibody or antigen, which is why the enzyme has to be linked to an appropriate antibody. This linking process was independently developed by Stratis Avrameas and G.B. Pierce.[6] Since it is necessary to remove any unbound antibody or antigen by washing, the antibody or antigen has to be fixed to the surface of the container; i.e., the immunosorbent has to be prepared. A technique to accomplish this was published by Wide and Jerker Porath in 1966.[7] In 1971, Peter Perlmann and Eva Engvall at Stockholm University in Sweden, and Anton Schuurs and Bauke van Weemen in The Netherlands independently published papers that synthesized this knowledge into methods to perform EIA/ELISA.[8][9]

非神經(jīng)元性烯醇化酶(NNE)由于各種抗原成份，包括小分子的半抗原，均可用以制備特異性的抗血清或單克隆抗體，利用此抗體作為試劑就可檢測標(biāo)本中相應(yīng)的抗原，因此免疫測定的應(yīng)用范圍極廣，在臨床檢驗中可用于測定：1) 體液中的各種蛋白質(zhì)，包括含量極少的蛋白質(zhì)如甲胎蛋白等。2) 激素，包括小分子量的甾體激素等。3) 抗生素和藥物。4) 病原體抗原，HBsAg、HBeAg等。5) 另外，也可利用純化的抗原檢測標(biāo)本中的抗體，例如抗-HBs等。

非神經(jīng)元性烯醇化酶(NNE)

解整合素樣金屬蛋白酶9(ADAM9)

未磷酸化類胰島素生長因子結(jié)合蛋白-1

NOD樣受體(NLR)

免疫球蛋白輕鏈lambda(λ-IgLC)

超敏前列腺特異性抗原(PSA-U S)

血管生成素4(ANG-4)

低密度脂蛋白受體相關(guān)蛋白6(LRP-6)

抗神經(jīng)元核抗體1型/抗Hu抗體(ANNA-1/Hu)

周期素依賴性激酶5(CDK5)

抗磷脂酰*抗體(APSA)

腫瘤壞死因子β(TNF-β)

抗角蛋白抗體(AKA)