轉(zhuǎn)錄因子Gli3抗體,Anti-Gli3抗體說(shuō)明書 >>>>點(diǎn)擊查看
Rabbit Anti-Gli3 antibody
Catalog Number : BYK- 11565R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: It has long been known that the overexpression of either Wnt-1 or the GLI proteins results in cancer; however, the molecular basis for this transformation was poorly understood. The Wnt-1 and GLI proteins have now been placed in a signaling cascade downstream of the mammalian homologs of the Drosophila hedgehog and patched proteins. The Drosophila segment polarity gene hedgehog （hh） encodes a secreted protein that appears to function in embryonic and imaginal disc patterning. The ptc gene, also identified as a Drosophila segment polarity gene, encodes the transmembrane protein patched, the expression of which is precisely regulated during embryonic development. Hedgehog has been shown to enhance the expression of the Wnt family of proteins through a signaling cascade involving the GLI transcription factors, while patched functions as a repressor opposing the effects of hedgehog. Mutations in the ptc gene, which result in unregulated hedgehog signaling, have been correlated with the most common type of cancer, basal cell carcinoma, which affects 750,000 individuals annually in the United States alone.
Specificity :
– Anti- Gli3 is a rabbit polyclonal antibody unconjugated
– specific for Gli3 of hu,mo
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 83kDa
轉(zhuǎn)錄因子Gli3抗體,Anti-Gli3抗體說(shuō)明書 Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
轉(zhuǎn)錄因子Gli3抗體,Anti-Gli3抗體說(shuō)明書 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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