儀器設(shè)備
ELISA試劑盒酶聯(lián)免疫檢測儀（Bio－550）；酶標(biāo)板 （96孔）；微量注射器。
2、試劑
（1）HRP標(biāo)記羊抗兔Ig G（1:1 000，國產(chǎn)）；標(biāo)準(zhǔn)牛IgG；兔抗牛血清（1:256）；
（2）包被液：0.05mol/L pH9.6碳酸緩沖液,4℃，保存, ELISA試劑盒Na2CO3 0.15g, NaHCO3 0.293g,蒸餾水稀釋至100 mL。
（3）稀釋液與洗滌液：0.01mol/LpH7.4 PBS--Tween--20, ４℃，保存。NaCl 8g, KH2PO4 0.2g, Na2HPO4·12H2O 2.9g, Tween—20，0.5mＬ。蒸餾水加至1000mＬ。
（4）封閉液：含0.1%明膠0.01mol/L PBS， pH7.4。
（5）鄰苯二胺底物溶液（臨用前配制）：臨用前將Na2HPO4·12H2O 2.9g,檸檬酸0.51g溶于100mL蒸餾水中, 再加入40mgOPD，40μL30%H2O2。
（6）ELISA試劑盒終止液：2mol/LH2SO4。于500mL蒸餾水中加入98%濃硫酸76mL混均即可。
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