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Catalog Number :HZ65104 Quantity size : 0.1ml （dilute with pH 7.4

0.01 M PBS or antibody diluent ）

Background: Insulin is a secreted peptide hormone that elicits metabolic effects such

as increases in glucose uptake and glycogen synthesis leading to a decrease in blood

glucose concentration. Insulin is first formed as a precursor molecule, preproinsulin,

which is later cleaved to proinsulin and finally to the mature Insulin hormone. Mature

Insulin consists of 51 amino acids, contained within an A chain and a B chain that are

connected by two disulfide bridges. It increases cell permeability to monosaccharides,

amino acids and fatty acids Insulin is secreted by the pancreas at basal levels in the

absence of exogenous stimuli, with secretion increasing in response to glucose.

Insulin action is effected by the binding of Insulin to cell-surface receptors on the

target cell membrane. Defects of Insulin are the cause of hyperproinsulinemia and of

type 2 diabetes mellitus.

specificity :

– Anti-Insulin is a Goat polyclonal antibody unconjugated.

– specific for Insulin of rabbit,human,rat

– use for western blotting,elisa,immunoprecipitation and immunohistochemistry

– Protein G affinity chromatography purification, purity :>95%

– Isotype: IgG

– mol wt: 12kDa

Application :

– Western blotting 1:100-500

– Immunohistochemistry 1:100-500

– ELISA 1:500-1000

– Optimal working dilutions must be determined by the end user.

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The

lyophilized antibody is stable at room temperature for at least one month and for

greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M

PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for

use in human, therapeutic or diagnostic applications.
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