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【文獻(xiàn)標(biāo)題】Effects of pamidronate disodium on the loss of osteoarthritic subchondral bone and the expression of cartilaginous and subchondral osteoprotegerin and RANKL in rabbits

【作者】You Lv1, Jie-yun Xia, Jing-yang Chen,et.al

【作者單位】徐州醫(yī)科大學(xué)附屬醫(yī)院（ Affiliated Hospital of Xuzhou Medical University）

【文獻(xiàn)中引用產(chǎn)品】

試劑藏紅O

試劑固綠

【關(guān)鍵詞】Osteoarthritis, Pamidronate disodium, Subchondral bone, Cartilage, Osteoprotegerin (OPG), Receptor activator of nuclear factor-κ B ligand (RANKL)

【DOI】10.1186/1471-2474-15-370

【影響因子(IF)】2.35

【出版期刊】《BMC Musculoskeletal Disorders》

【產(chǎn)品原文引用】

Histology and OARSI score

Rabbits were euthanized [25] and the medial condyles of the femurs were fixed with 4% paraformaldehyde (Boster, Wuhan, China) overnight at 4°C on a shaker. Whole medial condyles were decalcified in 14% ethylenediaminetetraacetic acid for 5 days at 4°C on a shaker. After dehydration by gradient alcohol and infiltration by xylene and paraffin, cartilage samples were embedded in paraffin. Paraffin-embedded joints (4 μm) were sectioned on a sagittal plane. Samples were stained with safranin O (Hengyuan,Shanghai, China) and fast green (Hengyuan) using standard protocols. All sections were evaluated for histologic signs of cartilage degeneration using a modified Osteoarthritis Research Society International (OARSI) scoring system [26].

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