ELISA試劑盒染色過(guò)程是：

①將已入蒸餾水后的切片放入蘇木精水溶液中染色數(shù)分鐘。

②酸水及氨水中分色，各數(shù)秒鐘。

③流水沖洗1小時(shí)后入蒸餾水片刻。

④入70％和90％酒精中脫水各10分鐘。

⑤入酒精伊紅染色液染色2—3分鐘。

脫水透明：

染色后的切片經(jīng)純酒精脫水，再經(jīng)二甲苯使切片透明。

ELISA試劑盒封固：

將已透明的切片滴上加拿大樹膠，蓋上蓋玻片封固。待樹膠略干后，貼上標(biāo)箋，ELISA試劑盒切片標(biāo)本就可使用。1號(hào)染色體開放閱讀框85(C1orf85)重組蛋白    Recombinant Chromosome 1 Open Reading Frame 85 (C1orf85)
POTE錨蛋白域家族成員G(POTEG)重組蛋白    Recombinant POTE Ankyrin Domain Family, Member G (POTEG)
P選擇素(SELP)重組蛋白    Recombinant Selectin, Plaet (SELP)
P-選擇素糖蛋白配體1(PSGL1)重組蛋白    Recombinant P-Selectin Glycoprotein Ligand 1 (PSGL1)
RAD23同源物B(RAD23B)重組蛋白    Recombinant RAD23 Homolog B (RAD23B)
RAD51同源物(RAD51)重組蛋白    Recombinant RAD51 Homolog (RAD51)
RAS癌基因家族成員RAB37(RAB37)重組蛋白    Recombinant RAB37, Member RAS Oncogene Family (RAB37)
Ras相關(guān)C3肉毒菌毒素底物1(Rac1)重組蛋白    Recombinant Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)
RNA結(jié)合基元蛋白20(RBM20)重組蛋白    Recombinant RNA Binding Motif Protein 20 (RBM20)
RNA結(jié)合基元蛋白24(RBM24)重組蛋白    Recombinant RNA Binding Motif Protein 24 (RBM24)
Runt相關(guān)轉(zhuǎn)錄因子2(RUNX2)重組蛋白    Recombinant Runt Related Transcription Factor 2 (RUNX2)
R-脊椎蛋白1(RSPO1)重組蛋白    Recombinant R-Spondin 1 (RSPO1)
R-脊椎蛋白3(RSPO3)重組蛋白    Recombinant R-Spondin 3 (RSPO3)
S100鈣結(jié)合蛋白(S100)重組蛋白    Recombinant S100 Calcium Binding Protein (S100)
S100鈣結(jié)合蛋白A11(S100A11)重組蛋白    Recombinant S100 Calcium Binding Protein A11 (S100A11)
S100鈣結(jié)合蛋白A12(S100A12)重組蛋白    Recombinant S100 Calcium Binding Protein A12 (S100A12)
S100鈣結(jié)合蛋白A13(S100A13)重組蛋白    Recombinant S100 Calcium Binding Protein A13 (S100A13)
S100鈣結(jié)合蛋白B(S100B)重組蛋白    Recombinant S100 Calcium Binding Protein B (S100B)
Smad同源物7(Smad7)重組蛋白    Recombinant Mothers Against Decapentaplegic Homolog 7 (Smad7)
SPARC樣蛋白1(SPARCL1)重組蛋白    Recombinant SPARC Like Protein 1 (SPARCL1)
TGFβ誘導(dǎo)早期應(yīng)答基因1(TIEG1)重組蛋白    Recombinant TGF Beta Inducible Early Response Gene 1 (TIEG1)
Tolloid樣蛋白1(TLL1)重組蛋白    Recombinant Tolloid Like Protein 1 (TLL1)
Toll樣受體2(TLR2)重組蛋白    Recombinant Toll Like Receptor 2 (TLR2)
Toll樣受體4(TLR4)重組蛋白    Recombinant Toll Like Receptor 4 (TLR4)
Toll樣受體6(TLR6)重組蛋白    Recombinant Toll Like Receptor 6 (TLR6)
Toll樣受體9(TLR9)重組蛋白    Recombinant Toll Like Receptor 9 (TLR9)
T-細(xì)胞可誘導(dǎo)共刺激分子(ICOS)重組蛋白    Recombinant Inducible T-Cell Co Stimulator (ICOS)
T-細(xì)胞免疫調(diào)節(jié)因子1(TCIRG1)重組蛋白    Recombinant T-Cell, Immune Regulator 1 (TCIRG1)
UL16結(jié)合蛋白2(ULBP2)重組蛋白    Recombinant UL16 Binding Brotein 2 (ULBP2)
USP6氨基端樣蛋白(USP6NL)重組蛋白    Recombinant USP6 N-Terminal Like Protein (USP6NL)
V-Fos-FBJ骨肉瘤病毒癌基因同源物(FOS)重組蛋白    Recombinant V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS)
VGF神經(jīng)生長(zhǎng)因子誘導(dǎo)蛋白(VGF)重組蛋白    Recombinant VGF Nerve Growth Factor Inducible (VGF)