The AFS98 monoclonal antibody reacts with mouse colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), and CD115. CSF1R is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. In mice CSF1R is expressed by monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts. CSF1R is a receptor for CSF1 and CSF1 signaling through CSF1R regulates the proliferation and differentiation of cells in the monocytic lineage. The AFS98 antibody has been reported to deplete macrophages and block CSFR1 in vivo.

Isotype Rat IgG2a, κ

Recommended Isotype Control(s) InVivoMAb rat IgG2a isotype control, anti-trinitrophenol

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Not available or unknown

Reported Applications in vivo macrophage depletion

in vitro CSF1R neutralization

in vivo monocyte depletion

Flow cytometry

Western blot

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 µm filtration

Production Purified from tissue culture supernatant in an animal free facility

Purification Protein G

RRID AB_2687699

Molecular Weight 150 kDa

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

AFS98單克隆抗體與小鼠集落刺激因子1受體（CSF1R）（也稱(chēng)為巨噬細(xì)胞集落刺激因子受體（M-CSFR））和CD115發(fā)生反應(yīng)。CSF1R是一種單程I型膜蛋白，是血小板衍生生長(zhǎng)因子受體家族的成員。在小鼠中，CSF1R由單核細(xì)胞/巨噬細(xì)胞，腹膜滲出物細(xì)胞，漿細(xì)胞樣和常規(guī)樹(shù)突狀細(xì)胞以及破骨細(xì)胞表達(dá)。CSF1R是CSF1的受體，通過(guò)CSF1R的CSF1信號(hào)傳導(dǎo)調(diào)節(jié)單核細(xì)胞譜系中細(xì)胞的增殖和分化。據(jù)報(bào)道，AFS98抗體可消耗巨噬細(xì)胞并阻斷體內(nèi)CSFR1。

艾美捷InVivoMAb 抗小鼠 CSF1R (CD115)#BE0213基本參數(shù)：

同種型 大鼠IgG2a， κ

推薦的同種型對(duì)照 體內(nèi)MAb大鼠IgG2a同種型對(duì)照，抗三-硝-基-苯-酚

推薦的稀釋緩沖液 體內(nèi)純 pH 7.0 稀釋緩沖液

免疫原 不可用或未知

報(bào)告的應(yīng)用程序 體內(nèi)巨噬細(xì)胞耗竭

體外腦脊液1R中

和 體內(nèi)單核細(xì)胞去除

流式細(xì)胞術(shù)

蛋白質(zhì)印跡

配方 PBS，pH 7.0

不含穩(wěn)定劑或防腐劑

內(nèi)毒素 <2EU/mg （<0.002EU/μg）

通過(guò)LAL凝膠凝血測(cè)定法測(cè)定

純度 >95%

由 SDS-PAGE 決定

無(wú) 菌 0.2 μm 過(guò)濾

生產(chǎn) 從無(wú)動(dòng)物設(shè)施中的組織培養(yǎng)上清液中純化

純化 蛋白 G

里德 AB_2687699

分子量 150 千達(dá)

存儲(chǔ) 抗體溶液應(yīng)以4°C的儲(chǔ)備濃度儲(chǔ)存。 不要凍結(jié)。

InVivoMAb 抗小鼠 CSF1R (CD115)部分文獻(xiàn)引用：

in vivo macrophage depletion

Kaminsky, L. W., et al. (2015). "Redundant Function of Plasmacytoid and Conventional Dendritic Cells Is Required To Survive a Natural Virus Infection" J Virol 89(19): 9974-9985.

in vitro CSF-R1 neutralization

Sheng, K. C., et al. (2014). "IL-3 and CSF-1 interact to promote generation of CD11c+ IL-10-producing macrophages" PLoS One 9(4): e95208.

in vivo monocyte depletion

Greter, M., et al. (2012). "GM-CSF controls nonlymphoid tissue dendritic cell homeostasis but is dispensable for the differentiation of inflammatory dendritic cells" Immunity 36(6): 1031-1046.