ImmunoChemistry艾美捷MitoPT JC-1測(cè)定利用熒光染料JC-1檢測(cè)線粒體膜去極化。當(dāng)積聚在帶負(fù)電的極化線粒體中時(shí)，JC-1發(fā)出橙色熒光。當(dāng)線粒體膜電位在凋亡或代謝應(yīng)激的細(xì)胞中崩潰時(shí)，JC-1試劑分散在細(xì)胞中并發(fā)出綠色熒光。使用流式細(xì)胞儀、熒光平板讀取器或熒光顯微鏡分析結(jié)果。

線粒體膜電位的喪失表明細(xì)胞凋亡。ICT的MitoPT JC-1測(cè)定利用電位熒光染料JC-1檢測(cè)線粒體膜通透性和膜去極化。JC-1染料具有分散在整個(gè)分子結(jié)構(gòu)中的離域正電荷。此外，其親脂性溶解性使其易于滲透膜并穿透活細(xì)胞。JC-1染料進(jìn)入帶負(fù)電荷的線粒體，在那里聚集并在激發(fā)時(shí)發(fā)出橙色熒光。當(dāng)?shù)蛲黾?xì)胞中線粒體膜電位崩潰時(shí)，MitoPT JC-1分布在整個(gè)細(xì)胞溶膠中。使用流式細(xì)胞儀、熒光平板讀取器或熒光顯微鏡分析結(jié)果。

ImmunoChemistry艾美捷MitoPT JC-1檢測(cè)試劑盒化學(xué)性質(zhì)：

試劑名稱JC-1

靶向線粒體去極化

激發(fā)/發(fā)射488 nm/590 nm和527 nm

分析方法流式細(xì)胞術(shù)、熒光顯微鏡、熒光板讀取器

樣品類(lèi)型細(xì)胞培養(yǎng)

儲(chǔ)存-20°C

試劑盒組分：

Kit 911: 400 Tests

MitoPT JC-1 Reagent, 100 Tests, #6260

10X Assay Buffer, 125 mL, #6259

CCCP, 50 mM, 600 µL, #6258

Kit Manual

Kit 924: 100 Tests

MitoPT JC-1 Reagent, 100 Tests, #6261

10X Assay Buffer, 60 mL, #685

CCCP, 50 mM, 125 µL, #6257

Kit Manual

ImmunoChemistry艾美捷MitoPT JC-1檢測(cè)試劑盒相關(guān)文獻(xiàn)：

Funakoshi, A;Honda, T;Ito, T;Tokura, Y. Cholecystokinin receptor antagonist suppresses melanoma growth by inducing apoptosis of tumor cells. JID Innovations. 2022 September 1; doi: 10.1016/j.xjidi.2022.100153. Abstract

Yoshioka, H;Komura, S;Kuramitsu, N;Goto, A;Hasegawa, T;Amizuka, N;Ishimoto, T;Ozasa, R;Nakano, T;Imai, Y;Akiyama, H. Deletion of Tfam in Prx1-Cre expressing limb mesenchyme results in spontaneous bone fractures. Journal of bone and mineral metabolism. 2022 August 10; doi: 10.1007/s00774-022-01354-2. Publication

Zhong, G;Long, H;Zhou, T;Liu, Y;Zhao, J;Han, J;Yang, X;Yu, Y;Chen, F;Shi, S. Blood-brain barrier Permeable nanoparticles for Alzheimer's disease treatment by selective mitophagy of microglia. Biomaterials. 2022 August 8; doi: 10.1016/j.biomaterials.2022.121690. Full Text

ImmunoChemistry Technologies簡(jiǎn)稱"ICT",提供各種細(xì)胞蛋白酶、細(xì)胞凋亡與細(xì)胞毒性檢測(cè)試劑盒等研究工具，同時(shí)包含高質(zhì)量高靈敏度的 ELISA開(kāi)發(fā)試劑。艾美捷科技是ImmunoChemistry的中國(guó)代理商，為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務(wù)。