Firefly Luciferase is widely used as a reporter gene to study gene regulation and function and drug screening. Due to the lack of endogenous activity in mammalian cells or tissues, it is a highly sensitive genetic reporter gene. However, the light generated by the reaction leads to the formation of suicidal adenosyloxyfluorescein on the surface of the enzyme. It results in a very short half-life of light emission with flashing dynamics. Because of this, flash type luminescence must be detected immediately after the reaction begins, which is incompatible with batch analysis of samples.

Steady Luc from Biotium ™  The Firefly HTS detection kit contains a proprietary mixture of substances that can prevent adenosine oxyfluorescein reactions and generate persistent luminescent signals. It generates a luminescent signal with a half-life of approximately 3 hours for high-throughput microplate detection.

Compared to flash type determination, Steady Luc ™  Isoluminescent Luciferase assay has low luminescence signal. The sensitivity and detection limit of this determination will depend on the expression level of Luciferase in your experimental system and the sensitivity of the photometer.

Biotium的Steady-Luc™HTS檢測(cè)系統(tǒng)是一種專有的混合物，可通過(guò)防止在酶表面形成腺苷酰氧化熒光素來(lái)改變酶促反應(yīng)，從而產(chǎn)生持久的信號(hào)（穩(wěn)定發(fā)光）。 它是一種同質(zhì)的高靈敏度螢火蟲(chóng)熒光素酶報(bào)告基因測(cè)定試劑盒，具有約3小時(shí)的半衰期，用于定量哺乳動(dòng)物細(xì)胞中的螢火蟲(chóng)熒光素酶表達(dá)，允許在微孔板中進(jìn)行批量處理。與閃光型檢測(cè)相比，發(fā)光型熒光素酶檢測(cè)（如Steady-Luc™）的發(fā)光信號(hào)較低。 檢測(cè)的靈敏度和極限將取決于實(shí)驗(yàn)系統(tǒng)中的熒光素酶表達(dá)水平以及光度計(jì)靈敏度。

Biotium還提供Steady-Luc™Firefly HTS檢測(cè)試劑盒（凍干），凍干檢測(cè)緩沖液便于室溫運(yùn)輸和-20°C儲(chǔ)存。

艾美捷Firefly HTS檢測(cè)試劑盒#BTM-30028-1組件：

D-熒光素

Steady-Luc™ 螢火蟲(chóng)檢測(cè)緩沖液

Firefly HTS檢測(cè)試劑盒屬性：

化驗(yàn)類型/選項(xiàng)：高通量分析、均質(zhì)分析、發(fā)光（發(fā)光型）

檢測(cè)方法/讀數(shù)：微孔板讀取器（發(fā)光）

儲(chǔ)存條件：在-60°C或更低溫度下儲(chǔ)存

Firefly HTS檢測(cè)試劑盒是一種均相、高靈敏度的螢火蟲(chóng)熒光素酶報(bào)告基因檢測(cè)試劑盒。它是一種發(fā)光型檢測(cè)，信號(hào)半衰期約為 3 小時(shí)，可在微孔板中進(jìn)行批處理。

特征：

簡(jiǎn)單的單步均相測(cè)定

發(fā)光半衰期約3小時(shí)

物超所值：與其他品牌相比節(jié)省高達(dá)45%