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當(dāng)前位置:武漢艾美捷科技有限公司>>技術(shù)文章>>中英文介紹丨艾美捷NEUROMAG 200 磁轉(zhuǎn)染試劑
NeuroMag Transfection Reagent is the first dedicated Magnetofection™ transfection reagent for neurons from 1 DIV to 21 DIV. It has proven to be extremely efficient in transfecting a large variety of primary neurons such as cortical, hippocampal, dorsal root ganglion and motor neurons with all types of nucleic acids. Moreover, high transfection efficiency was also achieved in primary astrocytes, oligodendrocyte precursors or neural stem cells as well as other cell lines (C6, B65, PC12, N2A...). Due to its unique properties, NeuroMag allows following transfected neurons during several days.
Great efficiency, ideal for primary neurons
Efficient from 0 DIV to 21 DIV
Non toxic and completely biodegradable: high transfected neurons viability
Ready-to-use, straightforward and rapid
For all types of nucleic acids
艾美捷NEUROMAG 200 磁轉(zhuǎn)染試劑#NM50200是一款專用試劑磁感應(yīng)™用于 1 DIV 至 21 DIV 神經(jīng)元的轉(zhuǎn)染試劑。事實(shí)證明,它在用所有類型的核酸轉(zhuǎn)染各種原代神經(jīng)元(如皮質(zhì)、海馬、背根神經(jīng)節(jié)和運(yùn)動(dòng)神經(jīng)元)方面非常有效。此外,在原代星形膠質(zhì)細(xì)胞、少突膠質(zhì)細(xì)胞前體或神經(jīng)干細(xì)胞以及其他細(xì)胞系(C6、B65、PC12、N2A等)中也實(shí)現(xiàn)了高轉(zhuǎn)染效率。由于其獨(dú)-特的性質(zhì),NeuroMag允許在幾天內(nèi)跟蹤轉(zhuǎn)染的神經(jīng)元。
NEUROMAG 200 磁轉(zhuǎn)染試劑特點(diǎn):
效率高,是原代神經(jīng)元的理想選擇
從 0 DIV 到 21 DIV 的高效
無(wú)毒且完-全可生物降解:高轉(zhuǎn)染神經(jīng)元活力
即用型、簡(jiǎn)單、快速
適用于所有類型的核酸
NEUROMAG 200 磁轉(zhuǎn)染試劑結(jié)果示例:
圖1:用 NeuroMag 有效轉(zhuǎn)染的大鼠原代海馬神經(jīng)元。在1孔板中轉(zhuǎn)染3 μg pVectOZ-GFP和5.24 μL NeuroMag的大鼠原代海馬神經(jīng)元的代表性圖像。轉(zhuǎn)染后48h熒光下拍攝照片
圖2:表達(dá)GFP的小鼠皮質(zhì)神經(jīng)元(培養(yǎng)3周,磁轉(zhuǎn)染后2-3天)。圖像采集:配備A1激光掃描共聚焦系統(tǒng)和60倍Apo TIRF物體鏡頭的尼康Ti-E顯微鏡。結(jié)果由C. Charrier博士提供(Charrier C.等人,2012年,Cell,第149卷,第4期,923-935)。
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