丙二醛(MDA)elisa檢測(cè)試劑盒英文介紹：

Principle of the Assay：

This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are wash ed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

Precautions

1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended.

2. After opening and before using, keep plate dry.

3. Before using the kit, spin tubes and bring down all components to the bottom of tubes.

4. Storage TMB reagents avoid light.

5. Washing process is very important, not fully wash easily cause a false positive and high background.

6. Duplicate well assay is recommended for both standard and sample testing.

7. Don’t let microplate dry at the assay, for dry plate will inactivate active components on plate.

8. Don’t reuse tips and tubes to avoid cross contamination.

9. Please do not mix the reagents in different kits of our company. Do not mix reagents from other manufacturers.

10. To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.

艾美捷丙二醛(MDA)elisa檢測(cè)試劑盒#EKF60157檢測(cè)原理：

該試劑盒基于競(jìng)爭(zhēng)ELISA檢測(cè)方法。該試劑盒中提供的微量滴定板已預(yù)先涂有目標(biāo)在反應(yīng)過(guò)程中，樣品或標(biāo)準(zhǔn)品中的靶標(biāo)與固相載體上固定量的靶標(biāo)競(jìng)爭(zhēng)用于靶特異性的生-物-素化檢測(cè)抗體上的位點(diǎn)。洗滌過(guò)量的綴合物和未結(jié)合的樣品或標(biāo)準(zhǔn)品并將HRP鏈親和素（SABC）加入每個(gè)微孔板孔中并孵育。然后TMB基板溶液添加到每個(gè)井中。通過(guò)加入硫酸溶液和顏色變化終止酶底物反應(yīng)在450nm的波長(zhǎng)下用分光光度法測(cè)量。然后通過(guò)以下方式確定樣品中目標(biāo)的濃度將樣品的OD與標(biāo)準(zhǔn)曲線進(jìn)行比較。

丙二醛(MDA)elisa檢測(cè)試劑盒操作步驟：

步驟1：在預(yù)涂板上分別設(shè)置標(biāo)準(zhǔn)、試樣、對(duì)照（空白）孔，然后記錄它們的位置。

第2步：在每個(gè)孔中加入50ul標(biāo)準(zhǔn)品或樣品。立即向每個(gè)孔中加入50ul生-物-素標(biāo)記的抗體，輕輕敲擊

平板以確保充分混合，然后在37°C下孵育45分鐘。

清洗步驟：抽吸并清洗盤子3次。

步驟3：向每個(gè)孔中加入100ul SABC工作溶液，并在37°C下孵育30分鐘。

清洗步驟：抽吸并清洗盤子5次。

第4步：添加90ul TMB基質(zhì)溶液。在37°C下培養(yǎng)10-20分鐘。

步驟5：添加50ul停止溶液。在450nm下立即讀取并計(jì)算

注意事項(xiàng)：

1.為了檢驗(yàn)實(shí)驗(yàn)操作的有效性和樣品稀釋比例的適當(dāng)性，建議使用標(biāo)準(zhǔn)品和少量樣品進(jìn)行中試。

2.打開(kāi)后和使用前，請(qǐng)保持盤子干燥。

3.在使用試劑盒之前，旋轉(zhuǎn)試管，并將所有組件降到試管底部。

4.TMB試劑應(yīng)避光保存。

5.洗滌過(guò)程非常重要，不完-全洗滌容易造成假陽(yáng)性和高背景。

6.對(duì)于標(biāo)準(zhǔn)測(cè)試和樣品測(cè)試，建議使用雙孔分析法。

7.在測(cè)定時(shí)不要讓微孔板干燥，因?yàn)楦稍锏钠桨鍟?huì)使平板上的活性成分失活。

8.不要重復(fù)使用尖-端和管道，以避免交叉污染。

9.請(qǐng)不要將試劑混合在我們公司的不同試劑盒中。不要混合其他制造商的試劑。

10.為了確保準(zhǔn)確的結(jié)果，在培養(yǎng)步驟中需要適當(dāng)?shù)卣掣狡桨迕芊鈩?/span>