NeuroMag Transfection Reagent is the first dedicated Magnetofection™ transfection reagent for neurons from 1 DIV to 21 DIV. It has proven to be extremely efficient in transfecting a large variety of primary neurons such as cortical, hippocampal, dorsal root ganglion and motor neurons with all types of nucleic acids. Moreover, high transfection efficiency was also achieved in primary astrocytes, oligodendrocyte precursors or neural stem cells as well as other cell lines (C6, B65, PC12, N2A...). Due to its unique properties, NeuroMag allows following transfected neurons during several days.

Great efficiency, ideal for primary neurons

Efficient from 0 DIV to 21 DIV

Non toxic and completely biodegradable: high transfected neurons viability

Ready-to-use, straightforward and rapid

For all types of nucleic acids

NeuroMag轉(zhuǎn)染試劑是第1款專用試劑磁感應(yīng)™用于 1 DIV 至 21 DIV 神經(jīng)元的轉(zhuǎn)染試劑。事實證明，它在用所有類型的核酸轉(zhuǎn)染各種原代神經(jīng)元（如皮質(zhì)、海馬、背根神經(jīng)節(jié)和運動神經(jīng)元）方面非常有效。此外，在原代星形膠質(zhì)細胞、少突膠質(zhì)細胞前體或神經(jīng)干細胞以及其他細胞系（C6、B65、PC12、N2A等）中也實現(xiàn)了高轉(zhuǎn)染效率。由于其獨-特的性質(zhì)，NeuroMag允許在幾天內(nèi)跟蹤轉(zhuǎn)染的神經(jīng)元。

艾美捷OZ Biosciences神經(jīng)標記起始試劑盒#KC30800：效率高，是原代神經(jīng)元的理想選擇；從 0 DIV 到 21 DIV 的高效；無毒且完-全可生物降解：高轉(zhuǎn)染神經(jīng)元活力；即用型、簡單、快速；適用于所有類型的核酸。

神經(jīng)標記起始試劑盒#KC30800的應(yīng)用：

轉(zhuǎn)染所有類型的核酸：DNA、shRNA、siRNA、mRNA、寡核苷酸

適用于各種神經(jīng)細胞：

初級神經(jīng)元：海馬、皮質(zhì)、小腦顆粒、運動神經(jīng)元

神經(jīng)干細胞，胚胎DRG

神經(jīng)元細胞系：A172、B65、C6、KS-1、N2A、PC12、SH-SY5Y、SKN-BE2、T98G、U251、U87、YH-13...

推薦的FOR：轉(zhuǎn)染神經(jīng)元細胞（參見細胞轉(zhuǎn)染數(shù)據(jù)庫和引文數(shù)據(jù)庫）。

神經(jīng)標記起始試劑盒#KC30800的結(jié)果：

圖1：用 NeuroMag 有效轉(zhuǎn)染的大鼠原代海馬神經(jīng)元。在1孔板中轉(zhuǎn)染3 μg pVectOZ-GFP和5.24 μL NeuroMag的大鼠原代海馬神經(jīng)元的代表性圖像。轉(zhuǎn)染后48h熒光下拍攝照片

圖2：表達GFP的小鼠皮質(zhì)神經(jīng)元（培養(yǎng)3周，磁轉(zhuǎn)染后2-3天）。圖像采集：配備A1激光掃描共聚焦系統(tǒng)和60倍Apo TIRF物體鏡頭的尼康Ti-E顯微鏡。結(jié)果由C. Charrier博士提供（Charrier C.等人，2012年，Cell，第149卷，第4期，923-935）。