The 12(S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Fast, accurate, and reliable results are obtained with ready-to-use liquid color-coded reagents that reduce error.

Easy-to-use color-coded reagents and pre-coated plates reduce errors and save time in the lab

High throughput format, obtain results from up to 39 samples in duplicate in just 5 hours

Highly sensitive assay, measure as little as 146 pg/ml

Reliable and reproducible results day-after-day and lot-after-lot

Sensitivity: 146 pg/ml (range 195 - 50,000 pg/ml)

Assay Time: 5 hours

Applications: ELISA, Colorimetric detection

Application Notes: For the quantitative determination of 12(S)-HETE in culture supernatants and plasma from any species. Cited sample types include serum, renal interstitial fluid and tissue.

Wavelength: 405 nm

Species reactivity: Species independent

Use/Stability: Store all components at +4°C, except standard and conjugate at -20°C.

Shipping: Shipped on Blue Ice

Contents: GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution

12（S）-HETE對多形核白細(xì)胞和血管平滑肌細(xì)胞具有趨化性和化學(xué)動力學(xué)。它也作為血管緊張素II誘導(dǎo)的醛固酮產(chǎn)生的第二信使。

12(S)-HETE-ELISA試劑盒是一種比色競爭性酶免疫測定試劑盒，5小時內(nèi)即可獲得結(jié)果。在405 nm處讀取吸光度。使用易于使用的液體色碼試劑可獲得快速、準(zhǔn)確和可靠的結(jié)果，從而減少誤差。

艾美捷12(S)-HETE-ELISA試劑盒——靈敏的ELISA試劑盒能夠檢測來自任何物種的培養(yǎng)上清液和血漿中的12（S）-HETE。

12(S)-HETE-ELISA試劑盒化學(xué)性質(zhì)：

替代名稱：12（S）-氫過氧四烯酸二十碳四烯酸

靈敏度：146 pg/ml（范圍195-50000 pg/ml）

化驗時間：5小時

應(yīng)用：ELISA，比色檢測

應(yīng)用說明：用于定量測定任何物種培養(yǎng)上清液和血漿中的12（S）-HETE。引用的樣本類型包括血清、腎間質(zhì)液和組織。

波長：405 nm

物種反應(yīng)性：物種無關(guān)

使用/穩(wěn)定性：在+4°C下儲存所有成分，-20°C下標(biāo)準(zhǔn)和共軛物除外。

運(yùn)輸：藍(lán)冰運(yùn)輸

內(nèi)容：GxR IgG微量滴定板、綴合物、抗體、測定緩沖液、洗滌緩沖液濃縮液、標(biāo)準(zhǔn)品、pNpp底物、停止溶液

12(S)-HETE-ELISA試劑盒艾美捷特點：

1.易于使用的色碼試劑和預(yù)涂板減少了錯誤并節(jié)省了實驗室時間

2.高通量格式，僅需5小時即可獲得多達(dá)39份樣本的結(jié)果，一式兩份

3.高靈敏度測定，測量值低至146 pg/ml

4.日復(fù)一日、一批又一批可靠且可重復(fù)的結(jié)果

12(S)-HETE-ELISA試劑盒文獻(xiàn)參考：

12-Lipoxygenase Inhibition on Microalbuminuria in Type-1 and Type-2 Diabetes Is Associated with Changes of Glomerular Angiotensin II Type 1 Receptor Related to Insulin Resistance: H.Z. Xu, et al.; Int. J. Mol. Sci. 17, E684 (2016), Application(s): Measurement of HETE levels in rats, Abstract; Full Text

Cancer cell-derived 12(S)-HETE signals via 12-HETE receptor, RHO, ROCK and MLC2 to induce lymph endothelial barrier breaching: C.H. Nguyen, et al.; Br. J. Cancer 115, 364 (2016), Application(s): 12(S)-HETE assay, rabbit, Abstract; Full Text

12/15-Lipoxygenase metabolites of arachidonic acid activate PPARγ: a possible neuroprotective effect in ischemic brain: L. Sun, et al.; J. Lipid. Res. 56, 502 (2015), Application(s): ELISA, Abstract; Full Text

Lobatin b inhibits NPM/ALK and NF-κB attenuating anaplastic-large-cell-lymphomagenesis and lymphendothelial tumour intravasation: I. Kiss, et al.; Cancer Lett. 356, 994 (2015), Application(s): ELISA using MCF7 cell culture supernatants,