This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cGMP. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.
Alternative size available: ADI-901-013 (5x96 wells)
Sensitive – optional acetylation protocol increases sensitivity 10-fold (0.088 pmol/ml)
Rapid – reliable results in just 3 hours
Trusted – regularly cited in peer-reviewed publications
Reduce errors – ready-to-use color-coded reagents and pre-coated plates
Simple – efficient and well-established sample handling protocols
Reliable – consistent lot-to-lot performance
環(huán)GMP(cGMP)是由鳥苷酸環(huán)化酶對GTP的作用形成的,其水平通常比cAMP低10-100倍。一些激素,如乙酰膽-堿、胰島素和催產素,以及某些其他化學物質,如血清素和組胺,會導致cGMP水平升高。鳥苷酸環(huán)化酶的刺激劑,如血管擴張劑硝-普鈉、硝酸甘-油、硝酸鈉和一氧化氮(NO)也會刺激cGMP水平。
cGMP-ELISA試劑盒是一種用于定量測定細胞外cGMP的比色競爭免疫分析試劑盒。乙酰化可提高靈敏度(包括試劑)。在405 nm處讀取吸光度。提供的即用液體色碼試劑減少了誤差。
cGMP-ELISA試劑盒艾美捷化學參數(shù):
替代名稱:循環(huán)GMP
靈敏度:(非乙?;?span style="font-family: Calibri;">0.37pmol/ml;(乙?;?span style="font-family: Calibri;">0.088pmol/ml(范圍(非乙?;?span style="font-family: Calibri;">0.16-500pmol/ml;(乙酰化)0.16-100 pmol/ml)
化驗時間:3小時
應用:ELISA,比色檢測
應用說明:用于定量測定任何物種的培養(yǎng)上清液、血漿、血清、唾液和尿液中的cGMP。
波長:405 nm
物種反應性:物種無關
交叉反應性:環(huán)狀GMP(100%)和<0.001%:GMP、GTP、環(huán)狀AMP、AMP、ATP、環(huán)狀UMP、CTP
使用/穩(wěn)定性:在+4°C下儲存所有成分,-20°C下標準和共軛物除外。
運輸:藍冰運輸
內容:GxR IgG微量滴定板、綴合物、抗體、測定緩沖液2、洗滌緩沖液濃縮液、標準品、pNpp底物、停止溶液、乙酸酐、三乙胺
cGMP-ELISA試劑盒——用于細胞外cGMP定量的靈敏(0.088pmol/ml)比色試劑盒。
cGMP-ELISA試劑盒艾美捷特點:
1.敏感–可選乙?;桨笇㈧`敏度提高10倍(0.088 pmol/ml)
2.僅需3小時即可獲得快速可靠的結果
3.可信——定期在同行評審出版物中引用
4.減少錯誤–即用色碼試劑和預涂板
5.簡單、高效且成熟的樣本處理協(xié)議
6.可靠–一致的批量性能
cGMP-ELISA試劑盒文獻參考:
Resveratrol treatment may preserve the erectile function after radiotherapy by restoring antioxidant defence mechanisms, SIRT1 and NOS protein expressions: T.E. Sener, et al.; Int. J. Impot. Res. 30, 179 (2018), Application(s): Rat cavernosal tissue lysate, Abstract;
Guanylin and uroguanylin mRNA expression is increased following Roux-en-Y gastric bypass, but guanylins do not play a significant role in body weight regulation and glycemic control: M.L. Fernandez-Cachon, et al.; Peptides 101, 32 (2017), Abstract;
Sildenafil attenuates hypoxic pulmonary remodelling by inhibiting bone marrow progenitor cells: S. Favre, et al.; J. Cell. Mol. Med. 21, 871 (2017),
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