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當(dāng)前位置:武漢艾美捷科技有限公司>>技術(shù)文章>>中英文說明書:艾美捷肝細(xì)胞培養(yǎng)基試劑盒
Hepatocyte culture mdium was designed and tested for our rat hepatocyte. This medium includes two components: Hepatocyte basal medium (H2004-70A1) and Hepatocyte culture medium supplement (H2004-70A2). The hepatocyte culture medium is a serum-free medium supplemented with BSA and several recombinant growth factors. Each lot of this product is tested in cell culture to ensure its sterility. This product does not contain antibiotics.
Use of Hepatocyte Culture Medium for Culturing Rat Hapatocytes:
1. Thaw the supplement and add the entire volume (5ml) to the basal medium (500ml). Antibiotics may also be added if contamination is a concern.
2. Take the amount of hepatocyte culture medium needed and warm to 37°C in a water bath or incubator. Avoid frequent temperature change to the entire bottle of medium. The hepatocyte culture medium is stable for at least 1 month stored at 4°C.
艾美捷肝細(xì)胞培養(yǎng)基在培養(yǎng)大鼠單核細(xì)胞中的應(yīng)用:
1、解凍補(bǔ)充劑,將整個體積(5ml)加入基礎(chǔ)培養(yǎng)基(500ml)中。如果擔(dān)心污染,也可以添加抗生素。
2、取所需量的肝細(xì)胞培養(yǎng)基,在水浴或培養(yǎng)箱中加熱至37°C。避免對整瓶介質(zhì)進(jìn)行頻繁的溫度變化。肝細(xì)胞培養(yǎng)基在4°C下至少穩(wěn)定1個月。
艾美捷肝細(xì)胞培養(yǎng)基試劑盒儲存和穩(wěn)定性:
肝細(xì)胞基礎(chǔ)培養(yǎng)基在4°C下儲存,至少穩(wěn)定6個月。沉淀物顏色或外觀的變化可能表明變質(zhì)。
等級:分子生物學(xué)等級
裝運(yùn)溫度:藍(lán)色的冰
儲存溫度:4攝氏度
艾美捷肝細(xì)胞培養(yǎng)基試劑盒相關(guān)研究:
E3010-60G Endothelial Progenitor Cells, Human, Growth Medium Kit, BioAssay™ 1Kit
E3010-68G Endothelial Progenitor Outgrowth Cells, Human, Growth Medium Kit, BioAssay™ 1Kit
E3010-72G Endothelial Progenitor Outgrowth Cells, Mouse, Growth Medium Kit, BioAssay™ 1Kit
H2004-70A Hepatocyte Culture Medium Kit, BioAssay™ 1Kit
M3005-34A Mesenchymal Stem Cells, Mouse, Growth Medium Kit, BioAssay™ 1Kit
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