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當(dāng)前位置:武漢艾美捷科技有限公司>>技術(shù)文章>>中英文說明書丨Jackson正常兔血清
動(dòng)物實(shí)驗(yàn)是醫(yī)學(xué)科學(xué)發(fā)展的*學(xué)科。家兔又是動(dòng)物實(shí)驗(yàn)時(shí)的shou選動(dòng)物,為使動(dòng)物實(shí)驗(yàn)資料和科學(xué)性更加完善,接下來一起了解下艾美捷Jackson 正常兔血清。
艾美捷Jackson 正常兔血清產(chǎn)品可應(yīng)用于大多數(shù)免疫學(xué)實(shí)驗(yàn),用作封閉液或空白對(duì)照.
艾美捷Jackson 正常兔血清使用:
正常兔血清是以凍干粉形式提供的,本品需要先溶解配制成100%原液后再稀釋使用。溶解方法:用5 mL 蒸餾水(dH2O)充分溶解凍干粉(如果不澄清,可對(duì)其離心處理),此時(shí)得到的即是100%原液。然后按照1:20的比例用緩沖液,如PBS或PBST,稀釋到需要的5%工作液。也可以根據(jù)自身實(shí)驗(yàn)條件,稀釋到合適的工作液濃度。
艾美捷011-000-120正常兔血清化學(xué)性質(zhì):
全名:正常兔血清丨Normal Rabbit Serum
Cat#:011-000-120
規(guī)格:5ml
蛋白濃度(Protein concentration):60 mg/mL
緩沖液(Buffer):0.01 M磷酸鈉,0.25 M氯化鈉,pH 7.6
防腐劑(Preservative):0.05%die氮化鈉
運(yùn)輸與保存方法:冰袋運(yùn)輸。凍干粉保存于2-8℃。
Information
Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.
Usage
Physical State: Freeze-dried solid
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.
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