雞Ia抗原在B細(xì)胞上表達(dá)，B細(xì)胞是單核細(xì)胞/巨噬細(xì)胞譜系的一個(gè)亞群，有絲分裂原激活的T細(xì)胞。小鼠抗雞IgM二抗識(shí)別的分子在結(jié)構(gòu)上與小鼠Ia抗原和人類DR抗原相似。
艾美捷/小鼠抗雞IgM二抗應(yīng)用：
FC – Quality tested
IHC-FS – Reported in literature
ICC – Reported in literature
IP – Reported in literature
Block – Reported in literature
艾美捷/小鼠抗雞IgM二抗儲(chǔ)存：
1.純化(UNLB)抗體以0.5 mg純化免疫球蛋白的形式提供于1.0 mL硼酸鹽緩沖鹽水中，pH值為8.2。不添加防腐劑或含胺緩沖鹽。在2-8°C下儲(chǔ)存。
2.熒光素(FITC)結(jié)合物以0.5 mg/1.0 mL PBS/NaN3的形式提供。在2-8°C下儲(chǔ)存。
3.在1.0毫升PBS/NaN3中以0.5毫克的形式提供生丨物丨素(BIOT)結(jié)合物。在2-8°C下儲(chǔ)存。
4.R-藻紅蛋白(PE)結(jié)合物以0.1 mg/1.0 mL PBS/NaN3和穩(wěn)定劑的形式提供。在2-8°C的溫度下儲(chǔ)存。不要凍結(jié)
5.保護(hù)熒光色素結(jié)合物不受光照。如果按照指示儲(chǔ)存，試劑在標(biāo)簽上顯示的時(shí)間內(nèi)是穩(wěn)定的。
小鼠抗雞IgM二抗參考文獻(xiàn)：
1. Ewert DL, Munchus MS, Chen CH, Cooper MD. Analysis of structural properties and cellular distribution of avian Ia antigen by using monoclonal
antibody to monomorphic determinants. J Immunol. 1984;132:2524-30. (Immunogen, FC, ICC, IP, Block, Quail, Dove, Pigeon, & Duck Reactivity)
2. Wang X, Efr GF. Apoptosis in feathers of Smyth line chickens with autoimmune vitiligo. J Autoimmun. 2004;22:21-30. (IHC-FS)
3. O'Halloran EK, Oesterle EC. Characterization of leukocyte subtypes in chicken inner ear sensory epithelia. J Comp Neurol. 2004;475:340-60. (IHCFS)
4. Das SC, Nagasaka N, Yoshimura Y. Changes in the localization of antigen presenting cells and T cells in the utero-vaginal junction after repeated
artificial insemination in laying hens. J Reprod Dev. 2005;51:683-7. (IHC-FS)
5. Subedi K, Yoshimura Y. Expression of MHC class I and II in growing ovarian follicles of young and old laying hens, Gallus domesticus. J Poult Sci.
2005;42:101-9. (IHC-FS)
6. Yoshimura Y, Fukui T, Nishibori M, Isobe N. Effects of age and gonadal steroids on the localization of antigen presenting cells in the epididymis of the
male chicken, Gallus domesticus. J Reprod Dev. 2006;52:363-71. (IHC-FS)
7. Bakri Y, Sarrazin S, Mayer UP, Tillmanns S, Nerlov C, Boned A, et al. Balance of MafB and PU.1 specifies alternative macrophage or dendritic cell
fate. Blood. 2005;105:2707-16. (FC, ICC)
8. Niikura M, Kim T, Hunt HD, Burnside J, Morgan RW, Dodgson JB, et al. Marek's disease virus up-regulates major histocompatibility complex class II
cell surface expression in infected cells. Virology. 2007;359:212-9. (ICC)
9. Rautenschlein S, Subramanian A, Sharma JM. Bioactivities of a tumour necrosis-like factor released by chicken macrophages. Dev Comp Immunol.
1999;23:629-40. (FC)
10. Chrz?stek K, Piasecki T, Wieliczko A. Impact of CpG oligodeoxynucleotide stimulation on percentage of T and B cells in chicken. Pol J Vet Sci.
2013;16:551-4. (FC)
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