操作步驟
①ELISA試劑盒抗原包被：兔抗人IgG 作為抗原，用包被液l：8000稀釋，100μL/孔加入聚苯乙烯96孔反應(yīng)板中。4℃放置。
②洗滌：次日傾去凹孔內(nèi)的液體，洗滌液洗3次。
③封閉：加l00μL/孔封閉液，室溫放置0.5h.
④洗滌：用洗滌液洗3次。
⑤加待測樣品(一抗)：將含單克降抗體的細(xì)胞培養(yǎng)上清在另-塊板上用PBS 連續(xù)稀釋(按照1：2或1：10)，100μL /孔加到 已包被的板上，ELISA試劑盒每個樣品平行做兩份，PBS 或空白培養(yǎng)基作為陰性對照，已知樣品作為陽性對照。ELISA試劑盒加蓋37℃恒溫箱溫育 1~2h。
⑥洗滌：用洗滌液洗3次。
⑦加酶標(biāo)抗抗體：兔抗鼠IgG-HRP，用封閉液l ：8000稀釋，100μL／孔，加蓋37℃恒溫箱溫育1h。
⑧洗滌：用洗滌液洗5次，蒸餾水洗2次。
⑨顯色：加新鮮配制的底物溶液100μL/孔，室溫暗處放置5～30min，顯示藍(lán)色。
⑩ELISA試劑盒終止反應(yīng)、比色：加50μL/孔終止液。顏色變黃；用酶標(biāo)儀測定450nm 處各孔的吸光值，陽性反應(yīng)的zui大稀釋度為待測
樣品的效價。
小鼠磷酸化蛋白激酶B(P-PKB)ELISA Kit Mouse phosphorylated protein kinase B,P-PKB ELISA Kit
小鼠磷酸化表皮生長因子受體(pEGFR)ELISA Kit Mouse phosphorylated epidermal growth factor receptor,pEGFR ELISA kit
小鼠磷酸化氨基末端蛋白激酶(P-JNK)ELISA Kit Mouse phosphorylated c-Jun N-terminal kinase,P-JNK ELISA kit
小鼠*腦啡肽(Leu-ENK)ELISA Kit Mouse Leu-Enkephalin,Leu-ENK ELISA Kit
小鼠鏈球菌(SK)抗體(IgG)ELISA Kit Mouse streptococcal(SK)antibody(IgG)ELISA kit
小鼠*羥化酶(TH)ELISA Kit Mouse tyrosine hydroxylase,TH ELISA Kit
小鼠跨膜蛋白97(TMEM97/MAC30)ELISA Kit Mouse Transmembrane protein 97,TMEM97/MAC30 ELISA Kit
小鼠可溶性腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體(sTRAIL)ELISA Kit Mouse soluble tumor necrosis factor-related apoptosis inducing ligand,sTRAIL ELISA KIT
小鼠可溶性血栓調(diào)節(jié)蛋白(sTM)ELISA Kit Mouse Soluable Thrombomodulin,sTM ELISA Kit
小鼠可溶性髓系細(xì)胞觸發(fā)受體-1(sTREM-1)ELISA Kit Mouse sTREM-1 ELISA Kit
小鼠可溶性瘦素受體(sLR)ELISA Kit Mouse Leptin Soluble Receptor,sLR ELISA Kit
小鼠可溶性凝集素樣氧化低密度脂蛋白受體1(sLOX-1)ELISA Kit Mouse soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1(sLOX-1)ELISA Kit
小鼠可溶性肌球蛋白重鏈2(sMHC-2)ELISA Kit Mouse soluble myosin heavy chain 2,sMHC-2 ELISA Kit
小鼠可溶性肌球蛋白重鏈1(sMHC-1)ELISA Kit Mouse soluble myosin heavy chain 1,sMHC-1 ELISA Kit
小鼠可溶性補(bǔ)體激活產(chǎn)物SC5b9 ELISA Kit Mouse soluble terminal complement complex SC5b9 ELISA Kit
小鼠可溶性Toll樣受體9(sTLR9/sCD289)ELISA Kit Mouse Soluble Toll-like Receptor 9,sTLR9/sCD289 ELISA Kit
小鼠可溶性Toll樣受體4(sTLR4)ELISA Kit Mouse Soluble Toll-like Receptor 4,sTLR4 ELISA Kit
小鼠可溶性Toll樣受體2(sTLR2)ELISA Kit Mouse Soluble Toll-like Receptor 2,sTLR2 ELISA Kit
小鼠可溶性Endoglin(ENG/sCD105)ELISA Kit Mouse Soluble Endoglin,ENG/sCD105 ELISA Kit
小鼠抗中性粒/中心體抗體(ACA)ELISA Kit Mouse anti-centrol and centrosome antibody,ACA ELISA Kit
小鼠抗血小板抗體IgG/M/A(PA-IgG/M/A)ELISA Kit Mouse plaet antibodies IgG/M/A,PA-IgG/M/A ELISA Kit
小鼠抗小核糖核蛋白/Sm(snRNP/Sm)抗體ELISA Kit Mouse small nuclear ribonucleoprotein(snRNP/Sm)antibody ELISA kit
小鼠抗線粒體ADP/ATP載體(AAC)抗體ELISA Kit Mouse anti-mitochondrial ADP/ATP carrier(AAC)antibody ELISA kit
小鼠抗透明帶抗體IgG(aZP-IgG)ELISA Kit Mouse anti-zona pellucida antibody IgG,aZP-IgG ELISA Kit
小鼠抗雙鏈DNA抗體/天然DNA抗體(IgM)ELISA Kit Mouse anti-double stranded DNA antibody(IgM)ELISA Kit
小鼠抗雙鏈DNA抗體/天然DNA抗體(IgG)ELISA Kit Mouse anti-double stranded DNA antibody(IgG)ELISA KIT
小鼠抗人生長激素抗體(IgG)ELISA Kit Mouse anti-human growth hormone antibody IgG ELISA Kit
小鼠抗利尿激素/血管加壓素/*加壓素(ADH/VP/AVP)ELISA Kit Mouse antidiuretic hormone/vasopressin/arginine vasopressin,ADH/VP/AVP ELISA Kit
小鼠抗精子抗體(AsAb)ELISA Kit Mouse anti-sperm antibody,AsAb ELISA Kit
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