日本富士 流感抗原檢測金標試劑

廣州健侖生物科技有限公司

廣州健侖長期供應(yīng)各種流感檢測試劑，包括進口和國產(chǎn)的品牌，主要包括日本富士瑞必歐、日本生研、美國BD、美國NovaBios、美國binaxNOW、英國clearview、廣州創(chuàng)侖等主流品牌。

主要檢測：甲型流感病毒檢測試劑、乙型流感病毒檢測試劑、甲乙型流感病毒檢測試劑、A+B流感病毒檢測試劑盒、流感病毒抗原快速檢測卡、流感病毒抗體快速檢測試劑盒、流感快速檢測試劑 c1c2。

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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日本富士 流感抗原檢測金標試劑

【產(chǎn)品說明書】

【原理】 

流感病毒檢測卡以雙抗體夾心法為基礎(chǔ)，采用免疫層析金標記技術(shù)，快速檢測流感病毒。  

【試劑組成】 

1. 流感病毒快速檢測卡40片/盒    

2. 樣本稀釋液40管/盒      

3. 棉簽40支/盒  

【操作方法】 一、樣品制備： 

1. 本檢測卡采集樣品為眼、氣管分泌物。將棉簽插入分泌物zui多的部位，輕輕搖動棉簽，讓棉簽充分吸收分泌物。 

2. 將棉簽在稀釋液中充分攪拌并反復擠壓試管壁，讓分泌物充分溶解到稀釋液中，得到待檢樣品。 

3. 樣品一般須當即進行檢測，否則應(yīng)冷藏保存，超過24小時的，應(yīng)該冷凍保存。  

二、操作步驟： 

1. 使用前將試劑盒和樣品恢復至室溫。 

2. 將棉簽浸入裝有樣品稀釋液的試管，充分攪拌混勻后，用一次性滴管取上清液。

3. 取出檢測卡，開封后平放于桌面上，從滴管中緩慢而準確地逐滴加入2–3滴混合液。 

4. 加樣品液后，約30秒內(nèi)，紅色的液體從靠樣品孔的觀察窗邊緣涌出。朝另一方向流動。 

5. 五分鐘后判斷結(jié)果，超過三十分鐘的結(jié)果判讀無效。  

【結(jié)果判定】        

1. 陽性：在觀察孔內(nèi)，若對照線顯色，檢測線同時顯色，判為陽性(如圖一、圖二)。陽性結(jié)果表明檢測液

中含有流感病毒。 

2. 陰性：在觀察孔內(nèi)，若對照線顯色，而檢測線不顯色，判為陰性（如圖三）。陰性結(jié)果表明檢測液中不含流感病毒。 

3. 無效：在觀察孔內(nèi)，若對照線不顯色，則結(jié)果無效，建議重新測試(如圖四、圖五)。   

【注意事項】 

1. 本品如果購買時發(fā)現(xiàn)過期、破損、污染、無效的產(chǎn)品，請在購買處進行更換。  

2. 測試樣品來自動物，可能有潛在感染性，樣品和使用過的試劑應(yīng)被看作微生物危險品處理。

3. 所有檢測卡啟封后馬上使用，此前不要隨意打開。 

4. 本品為一次性產(chǎn)品，請勿二次使用；請勿使用非本品隨附的稀釋液。  

【儲存及有效期】 

1. 保存于干燥陰涼處（2~30℃），有效期為18個月，生產(chǎn)日期見外包裝盒。

2. 打開包裝后，在1小時內(nèi)使用。

【產(chǎn)品介紹】

日本富士 

存在環(huán)境核酸檢測試劑盒
軍團菌是隱藏在空調(diào)制冷裝置中的致病菌，隨冷風吹出浮游在空氣中，吸入人體后會出現(xiàn)上呼吸道感染及發(fā)熱的癥狀，嚴重者可導致呼吸衰竭和腎衰竭。軍團菌zui早是由于*室內(nèi)密閉作業(yè)，導致群體發(fā)生上呼吸道感染而得名。軍團病是一種非常嚴重的、有時可以致命的肺炎。軍團病是由軍團桿菌引起，這種細菌產(chǎn)生在自然環(huán)境中，在溫水里及潮熱的地方蔓延。人工供水系統(tǒng)有時也能為軍團桿菌的大量繁殖提供生存環(huán)境。這些系統(tǒng)包括淋浴器、礦泉池、噴泉以及空調(diào)設(shè)備的冷卻水塔。人們通常是由于呼吸了被軍團桿菌污染的水源散發(fā)的水霧而傳染上軍團病的。
鑒定方法核酸檢測試劑盒
1. 細菌培養(yǎng)法
軍團菌為革蘭氏陰性桿菌，專性需氧，胞內(nèi)寄生菌。軍團菌zui初是從以軍團菌病人肺組織感染的豚鼠中分離出來的。這種方法雖然有較高的可靠性，但非常昂貴，而且費時費力，不久就被平板培養(yǎng)法所取代核酸檢測試劑盒  。目前標準培養(yǎng)基為活性碳酵母浸膏瓊脂平板，也稱軍團菌生長平板(BCYE)。接種在 BCYE 平板上的樣品在溫度為35-37oC，培養(yǎng)10天，在濃度為2.5% CO2 的環(huán)境下培養(yǎng)更有利于軍團菌的生長。軍團菌的菌落通常呈白色、灰色、有熒光。軍團菌在 BCYE 平板上生長而在平板和半光氨酸缺失 BCYE-Cys 平板上不生長。
2.血清學玻片凝集法
軍團菌感染l周左右可檢測出血清中軍團菌特異性IgM抗體，2周左右可檢測到特異性IgG抗體。
現(xiàn)已發(fā)現(xiàn)嗜肺軍團菌有15個血清型。其中，嗜肺軍團菌血清1型（Lp1）與人類疾病關(guān)系zui密切，其次為血清4型（Lp4）和6型（Lp6）。
3.核酸擴增法
核酸擴增技術(shù)，即聚合酶鏈式反應(yīng)（polymerase chainreaction，PCR），其基本原理是設(shè)計、合成兩條寡核苷酸，作為引物，對應(yīng)于待測病原微生物某一段特異性序列的兩端，然后在體外模擬DNA體內(nèi)復制的過程反復擴增，使靶序列放大上萬倍甚至上百萬倍而被檢測出來。
常規(guī)PCR：鑒定標準是通過電泳來判斷是否有擴增的核酸片段以及擴增產(chǎn)物的大小是否正確。
實時熒光PCR：通過對實時熒光PCR反應(yīng)的每一個循環(huán)產(chǎn)物進行熒光信號的實時監(jiān)測來判斷分析。
殺滅方法核酸檢測試劑盒
溫度殺菌：水加熱三分鐘到至少70°。
化學殺菌：大多用氯，二氧化氯，氯化鈉，次氯酸鈣溶劑殺菌，在使用化學品之前必須先清洗水管。
紫外線殺菌：照射紫外線短波，波長254納米可以使病菌無法活躍，但可能存在于變形蟲里。為更有力的除菌可以在附加使用超聲波。
薄膜技術(shù)：越來越多的使用薄膜來去除微生物。一般可以利用薄膜的微孔讓直徑小于0.2微米的顆粒包括細菌，甚至部分病毒過濾掉。

診斷與治療核酸檢測試劑盒
軍團菌病是由軍團菌科細菌所致的肺部感染。多散在發(fā)病或小流行，亦可暴發(fā)流行。由空調(diào)、供水系統(tǒng)、霧化吸入污染的水源引起感染。本病特征為肺炎伴全身性毒血癥癥狀，嚴重者出現(xiàn)周圍回圈衰竭、呼吸衰竭。PCR檢測試劑盒X線表現(xiàn)在早期為單葉斑片狀陰影，繼而肺實變，病變迅速發(fā)展至多肺葉段，可有肺膿腫形成或少量胸腔積液征。確診依賴于特異性抗體的檢查及在痰、胸腔積液、肺組織活檢標本中分離出細菌。紅霉素、利福平對本病的治療效果較好。
臨床表現(xiàn)
1．緩慢起病，反復寒戰(zhàn)、高熱、伴頭痛、肌痛、乏力，常有腹痛、嘔吐、腹瀉和全身衰竭，多數(shù)病人有咳嗽、胸痛，少量粘液痰，可有血痰。嚴重患者有呼吸困難，紫紺，甚至呼吸衰竭，神經(jīng)精神癥狀，譫妄，定向力障礙等。
2．急性病容，呼吸急促。肺部有羅音和實變體征。約20%有相對緩脈。少數(shù)患者有胸膜摩擦音。
診斷依據(jù)
1．可為流行或散發(fā)發(fā)病，多發(fā)生于中老年或有慢性疾病患者。
2．發(fā)熱、大汗、咳嗽，咳白粘痰，伴胸痛、肌痛及乏力。神經(jīng)、精神癥狀或PCR檢測試劑盒、嘔吐、腹瀉，嚴重者可出現(xiàn)腎功能衰竭表現(xiàn)。
3．呼吸困難，相對緩脈。肺部可有濕羅音和實變體征或有胸膜摩擦音。
4．白細胞總數(shù)增高，肝功能可異常，血尿素氮增高。
5．PCR檢測試劑盒X線顯示單葉或多葉變化較迅速的片狀陰影，常伴有膿腫形成，胸腔積液征。
6．特殊檢查：直接熒光抗體試驗陽性。肺活檢組織用特殊染色軍團菌陽性； 間接熒光抗體試驗陽性。特異性血清抗體滴定>1:256，或雙份血清對比高4倍；放射免疫測定法或梅聯(lián)免疫吸附試驗測定尿中可溶性細菌抗原，痰、胸液和肺組織特殊培養(yǎng)有嗜肺軍團菌生長。
治療原則
1．抗菌療法。
2．對癥治療。
3．支持療法。

日本富士 

我司還提供其它進口或國產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌、食品安全、化妝品檢測、藥物濫用檢測等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

想了解更多的產(chǎn)品及服務(wù)請掃描下方二維碼：

【公司名稱】 廣州健侖生物科技有限公司
【市場部】    楊永漢

【】 
【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室

There is an environmental nucleic acid detection kit
Legionella is a pathogen hidden in air conditioners and refrigeration units. It floats in the air with cold air and causes upper respiratory tract infections and fever when inhaled. In severe cases, it causes respiratory failure and kidney failure. Legionella was originally the result of indoor airtight military operations, resulting in the group name after the upper respiratory tract infection. Legionnaires' disease is a very serious and sometimes fatal pneumonia. Legionnaires' disease is caused by Legionella bacteria, a bacteria that occurs in the natural environment and spreads in warm water and hot flashes. Artificial water systems can sometimes provide a living environment for the mass production of Legionella bacteria. These systems include showers, mineral pools, fountains and cooling towers for air-conditioning equipment. Legionnaires' disease is usually transmitted as a result of breathing in water mist from water contaminated with Legionella bacteria.
Identification methods Nucleic acid detection kit
Bacterial culture
Legionella is Gram-negative bacilli, obligate aerobic, intracellular parasites. Legionella was initially isolated from guinea pigs infected with lung tissue of Legionnaires' patients. Although this method has high reliability, but very expensive, but also time-consuming and laborious, and soon was replaced by plate culture nucleic acid detection kit. The current standard medium is activated carbon yeast extract agar plate, also known as Legionella growth plate (BCYE). The samples inoculated on BCYE plates grew at a temperature of 35-37oC for 10 days and cultured at a concentration of 2.5% CO2 more conducive to the growth of Legionella. Legionella colonies are usually white, gray, fluorescent. Legionella grew on BCYE plates and did not grow on plates and half-my-leucine-deficient BCYE-Cys plates.
2. Serological slide agglutination method
Legionella infection can be detected about 1 week in the serum of Legionella specific IgM antibodies, about 2 weeks to detect specific IgG antibodies.
Legionella pneumophila has been found in 15 serotypes. Among them, Legionella pneumophila serotype 1 (Lp1) has the closest relationship with human diseases, followed by Lp4 and Lp6.
3 nucleic acid amplification method
The principle of nucleic acid amplification, that is, polymerase chain reaction (PCR), is to design and synthesize two oligonucleotides, which serve as primers and correspond to the two ends of a specific sequence of the pathogenic microorganism to be tested. Then in vitro replication of DNA replication in vivo repeated amplification process, the target sequence to enlarge tens of thousands or even millions of times were detected.
Conventional PCR: The standard of identification is determined by electrophoresis to determine whether the amplified nucleic acid fragment and the size of the amplified product are correct.
Real-time fluorescence PCR: Real-time fluorescence PCR reaction through real-time monitoring of each signal to determine the fluorescence signal analysis.
Killing method Nucleic acid test kit
Temperature Sterilization: Water is heated for three minutes to at least 70 °.
Chemical sterilization: Mostly chlorine, chlorine dioxide, sodium chloride, calcium hypochlorite solvent sterilization, the use of chemicals must be cleaned before the water pipe.
UV disinfection: Irradiation UV shortwave, 254 nm wavelength can make bacteria can not be active, but may exist in amoeba. For more vigorous sterilization can be used in addition to ultrasound.
Thin film technology: More and more thin films are used to remove microorganisms. The micropores of the membrane can generally be used to filter particles smaller than 0.2 microns in diameter, including bacteria, and even some viruses.

Diagnosis and treatment of nucleic acid detection kit
Legionnaires' disease is a pulmonary infection caused by Legionella bacteria. More scattered in the incidence or pandemic, but also outbreaks. By the air conditioning, water supply system, atomized inhalation of contaminated water cause infection. The disease is characterized by symptoms of pneumonia with systemic toxemia, severe circulatory failure appears around, respiratory failure. X-ray PCR detection kit performance in the early single-leaf patchy shadow, followed by consolidation of the lungs, the rapid development of lesions to the lung segment, may have the formation of pulmonary abscess or a small amount of signs of pleural effusion. Confirmed diagnosis depends on the specific antibodies and sputum, pleural effusion, lung biopsy specimens isolated bacteria. Erythromycin, rifampin treatment of the disease better.
Clinical manifestations
1. Slow onset, chills, fever, with headache, myalgia, fatigue, often abdominal pain, vomiting, diarrhea and systemic failure, most patients have cough, chest pain, a small amount of mucus sputum, bloody sputum may have. Severe patients have dyspnea, cyanosis, and even respiratory failure, neuropsychiatric symptoms, delirium, disorientation and so on.
2. Acute illness, shortness of breath. The lungs have rales and consolidation signs. About 20% has a relatively slow pulse. A small number of patients have pleural friction.
Diagnose based on
1. For epidemic or sporadic incidence, occurred in middle-aged or elderly patients with chronic diseases.
2. Fever, sweating, cough, cough and white phlegm, with chest pain, myalgia and fatigue. Neurological, psychiatric symptoms or PCR test kit, vomiting, diarrhea, severe renal failure may occur.
3. Breathing difficulties, relative slow pulse. Lung may have wet rales and consolidation signs or pleural friction sound.
4. The total number of white blood cells increased, abnormal liver function, blood urea nitrogen increased.
5. PCR test kit X-ray showed single or multi-leaf changes more rapid patchy shadow, often accompanied by abscess formation, pleural effusion sign.
6. Special examination: direct fluorescent antibody test positive. Lung biopsy with special staining Legionella positive; indirect fluorescent antibody test was positive. Specific serum antibody titer> 1: 256, or double serum high contrast 4 times; radioimmunoassay or Mellitus Immunosorbent Assay Determination of urine soluble bacterial antigens, sputum, pleural fluid and lung tissue Special Legionella pneumophila Grow.
Treatment principles
1. Antibacterial therapy.
2. Symptomatic treatment.
3. Support therapies.