RIPA裂解液成分用量:
Tris(MW121.14, PH7.5):3.02g(50mM)
NaCl:4.38g(150mM )
TritonX-100:5ml(1%)
脫氧膽酸鈉:5g(1%)
SDS:0.5g(0.1%)
蒸餾水:定容至500ml
RIPA裂解液配制方法:
按配方稱取各成分,先用300ml蒸餾水溶解
調(diào)pH值至7.5
用蒸餾水定容至500ml
混勻后4℃保存,長期保存于-20℃
使用時(shí),加入蛋白酶抑制劑(protease inhibitor)cooktail
22Rv1細(xì)胞 熱銷細(xì)胞:1301細(xì)胞,22Rv1細(xì)胞,293細(xì)胞,293Ad5細(xì)胞,293 Cells細(xì)胞,293T細(xì)胞,293T/17細(xì)胞,2BS細(xì)胞
5637(HTB-9)細(xì)胞,6T-CEM細(xì)胞,769-P細(xì)胞,786-O細(xì)胞,95-D細(xì)胞,A172細(xì)胞,A-204細(xì)胞,A2780細(xì)胞,
A3細(xì)胞,A-375細(xì)胞,A-427細(xì)胞,A-431細(xì)胞,A498細(xì)胞,A549細(xì)胞,A-673細(xì)胞,A875 LIFE細(xì)胞,
AAV-293細(xì)胞,ACC-2細(xì)胞,ACC-3細(xì)胞,ACHN細(xì)胞,AE1201細(xì)胞,AGS細(xì)胞,AN3CA細(xì)胞