mouse Anti-Bcl-2 抗體現(xiàn)貨
From:abcam
Catalog Number : RS-0032R
Quantity size : 100ul (dilute with pH 7.4 0.01 M PBS or antibody diluent )
background:
The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8).
Function:
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Specificity : Anti-Bcl-2 is a mouse monoclonal antibody
specific for anti-Bcl-2 of human,mouse,Rat, Dog, Pig, Cow, Rabbit
- use for western blotting,elisa,immunoprecipitation and immunohistochemistry
- Protein G affinity chromatography purification, purity :>95%Anti-Bcl-2 抗體現(xiàn)貨
- Isotype: IgG
- mol wt: 26kDa
Application :
- WB=1:100-500
- ELISA=1:500-1000
- IHC-P=1:100-500
- IHC-F=1:100-500
- optimal dilutions/concentrations should be determined by the end user.
KLH conjugated synthetic peptide derived from human Bcl-2
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃Anti-Bcl-2 抗體現(xiàn)貨
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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