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技術(shù)文章

Mouse Anti-ERK antibody說明書

閱讀:268發(fā)布時間:2014-8-23

Mouse Anti-ERK antibody

From:ABCAM

Catalog Number :ab119933

Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent ) Background: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4(HSF4) (By similarity). Highest levels within the nervous system, expressed in different tissues, mostly in intestine, placenta and lung. Increased expression during development. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Specificity : · Anti-ERK is a mouse monoclonal antibody unconjugated

Mouse Anti-ERK antibody · specific for anti-ERK of Rat ,Human

· use for western blotting,elisa,immunoprecipitation and immunohistochemistry

· Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt44kDa

Application :

· Western blotting: 1:100-500

· Immunohistochemistry: 1:100-500

· ELISA: 1:500-1000

· Optimal working dilutions must be determined by the end user.


optimal dilutions/concentrations should be determined by the end user..

Storage: Store at -20 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4

 

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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