Streptavidin/HRP
Catalog Number :Rs-90437
Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
Background:
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin
molecule with a KD of ~1x10-15M. The preparation contains an N- and C-terminal shortened
variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance
towards proteolytic degradation and accessibility of the biotin binding pocket as compared to
native streptavidin. The high affinity recognition of biotin and biotinylated molecules has made
streptavidin one of the most important components in diagnostics and laboratory kits.
This enzyme conjugate solution can be used to stain biotinylated antibodies.
Application :
– WB=1:100-500 ELISA=1:1000-10000 IP=1:20-100 IHC-P=1:100-500
IHC-F=1:100-500 IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user
Storage: Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. The
lyophilized antibody is stable at room temperature for at least one month and for
greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M
PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for
use in human, therapeutic or diagnostic applications.
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