Anti-EDG2/LPA1
From:ABCAM
Catalog Number :RS-2880R
Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent ) Background: EDG2 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. There are eight known members of the EDG receptor family and they are implicated in mediating growth-related effects such as induction of cellular proliferation, alterations in differentiation and survival, and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C, and several proteins not used within 12 hours. Edg2 has been reported in most human tissues, and is especially abundant in brain cortical regions. ESTs have been isolated from bone, brain, breast, connective tissue, embryo, heart/melanocyte/uterus, lung, prostate and uterus libraries.
Function : Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.
Subunit : Interacts with RALA and ADRBK1.
Subcellular Location : Cell surface. Cell membrane; Multi-pass membrane protein. Note=Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles.Tissue Specificity : Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes.
Similarity : Belongs to the G-protein coupled receptor 1 family.Database links : UniProtKB/Swiss-Prot: Q92633.3
.Specificity : Anti -EDG2/LPA1 is a rabbit polyclonal antibody
specific for EDG2/LPA1of Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Sheep,
use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 41kDa
Application :
– WB=1:100-500 ELISA=1:2000-5000 IP=1:100-500 IHC-P=1:100-500 IHC-F=1:30-200 ICC=1:30-200 IF=1:30-200
– KLH conjugated synthetic peptide derived from human EDG2/LPA1
– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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