Rabbit Anti-Ki67抗體說明書現(xiàn)貨
Catalog Number : ab15580
Quantity size : 100ug （dilute with pH 7.4 0.01 M PBS or antibody diluent ） Background: Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle （G1, S, G2 and M phase）. It is absent in resting （G0） cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms （immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index）. In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Function : Thought to be required for maintaining cell proliferation.
Specificity : Anti- KI67 is a rabbit polyclonal antibody
–specific for KI67 of Mouse, Rat, Horse, Cow, Dog, Human, Pig, Indian Muntjac, Monkey, Chinese Hamster, Marmoset （common）, Syrian Hamster
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
–Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 358kDa
Application :
- WB=1:100-500
- ELISA=1:500-1000
- IHC-P=1:100-500
- IHC-F=1:100-500
- IF=1:100-500
– KLH conjugated synthetic peptide derived from human IL-8 C-terminus
– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
