Rabbit Anti-Skp2 p45
Catalog Number : HZ773961 Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
Background: The critical role that the family of regulatory proteins known as cyclins plays in eukaryotic cell cycle regulation is well established. The best characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of MPF (maturation promoting factor). Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.
pecificity :
–Anti-Skp2 p45 is a rabbit polyclonal antibody unconjugated
–specific for Skp2 p45 of human, mouse, rat
–use for western blotting,elisa,immunoprecipitation and immunohistochemistry
–Protein G affinity chromatography purification, purity :>95%
–Isotype: IgG
–mol wt: 45kDa
Application :
–Western blotting 1:100-500
–Immunohistochemistry 1:100-500
–ELISA 1:500-1000
–Optimal working dilutions must be determined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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