EJ/GlyRS

試劑盒名稱:吡哆醛/吡哆醇*6磷酸酶(PDXP)ELISA試劑盒

英文名：Human anti-EJ-antibody，EJ/GlyRS

品牌:BIOFINE
種屬:大鼠ELISA試劑盒
檢測波長:450nm
所需樣本體積: 50-100ul
適用范圍:僅供科研
保存及有效期:2-8℃，六個月，-20℃一年
檢測目的:用于測定血清，血漿及相關液體吡哆醛/吡哆醇*6磷酸酶(PDXP)含量。適合檢測包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細胞培養(yǎng)上清、組織勻漿等標本。 

注意事項：1. 當混合蛋白溶液時應盡量輕緩，避免起泡。2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。3. 一次加樣時間控制在5分鐘內(nèi)，如標本數(shù)量多，*使用排槍加樣。4. 請每次測定的同時做標準曲線，做復孔。5. 如標本中待測物質(zhì)含量過高，請先稀釋后再測定，計算時請zui后乘以稀釋倍數(shù)。6. 在配制標準品、檢測溶液工作液時，請以相應的稀釋液配制，不能混淆。7. 底物請避光保存。8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

吡哆醛/吡哆醇*6磷酸酶(PDXP)contains the standard concentration of analyte will be prepared. Unknowns that generate a signal that is stronger than the known sample are "positive." Those that generate weaker signal are "negative." Doctor Dennis E Bidwell and Alister Voller created the test. History Before the development of the ELISA, the only option for conducting an immunoassay was radioimmunoassay, a technique using radioactively-labeled antigens or antibodies. In radioimmunoassay, the radioactivity provides the signal, which indicates whether a specific antigen or antibody is present in the sample. Radioimmunoassay was first described in a paper by Rosalyn Sussman Yalow and Solomon Berson published in 1960.[5] Because radioactivity poses a potential health threat, a safer alternative was sought. A suitable alternative to radioimmunoassay would substitute a non-radioactive signal in place of the radioactive signal. When enzymes (such as peroxidase) react with appropriate substrates (such as ABTS or 3,3’,5,5’-Tetramethylbenzidine), a change in color occurs, which is used as a signal. However, the signal has to be associated with the presence of antibody or antigen, which is why the enzyme has to be linked to an appropriate antibody. This linking process was independently developed by Stratis Avrameas and G.B. Pierce.[6] Since it is necessary to remove any unbound antibody or antigen by washing, the antibody or antigen has to be fixed to the surface of the container; i.e., the immunosorbent has to be prepared. A technique to accomplish this was published by Wide and Jerker Porath in 1966.[7] In 1971, Peter Perlmann and Eva Engvall at Stockholm University in Sweden, and Anton Schuurs and Bauke van Weemen in The Netherlands independently published papers that synthesized this knowledge into methods to perform EIA/ELISA.[8][9]

吡哆醛/吡哆醇*6磷酸酶(PDXP)

細胞間粘附分子2(ICAM-2/CD102)

骨保護素(OPG)

心肌特異性肌鈣蛋白T(cTnT)

降鈣素基因相關肽(CGRP)

5脂加氧酶(5-LO/LOX)

兒茶酚胺(CA)

肽聚糖(PG)

毒蕈堿型乙酰*受體(M-AChR)

酸性磷酸酶(ACP)

低密度脂蛋白免疫復合物(LDL-IC)

雙氫睪酮(DHT)

蛋白激酶A(PKA)